货号：06-821

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-PKCε (Ser729) Antibody | 06-821 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes PKCε phosphorylated on serine residue 729. View All» Molecular Weight: 95 kDa View All» Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 726-735 (CKGF[pS]YFGEDL) of human PKCε. The sequence is identical in mouse, rat and rabbit. View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Background Information: Protein Kinase C epsilon (PKC epsilon) is an ~87 kDa (migrates predominantly as 95 kDa in quiescient cells) member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC epsilon is an actin cytoskeleton regulating protein whose overexpression confers an oncogenic phenotype by affecting the ras signaling cascade. PKC epsilon is phosphorylated by PDK1 on threonine 566 and is then autophosphorylated on the turn motif threonine 710 and the hydrophobic motif serine 729. Catalytically inactive PKC epsilon is not phosphorylated on serine 729. Phosphorylation of serine 729 is mediated by mTOR-dependent pathways, plays an important role in regulating PKC epsilon subcellular localization, and is responsible for the appearance of the 92 kDa PKC epsilon species. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Rabbit
  View All» Control: Included Positive Antigen Control: Catalog #12-305, 3T3/A31 Cell Lysate. Add 2.5 μL of 2-mercaptoethanol/100 μl of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Evaluated by western blot on RIPA lysates from 3T3/A31 cells.Western Blot Analysis: 0.5-2 μg/mL of this antibody detected phosphorylated PKCε (Ser729) in RIPA lysates from 3T3/A31 cells. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbie polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at –20°C. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» UniProt Number: Q02156 View All» Entrez Gene Number: NM_005400 View All» Gene Symbol:
  • PRKCE

  • MGC125656

  • PKCE

  • MGC125657

  • nPKC-epsilon
    View All» Alternate Names: protein kinase C, epsilon View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. ... View All» UniProt Summary: FUNCTION: SwissProt: Q02156 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.SIZE: 737 amino acids; 83674 Da SUBUNIT: Forms a ternary complex with TRIM63 and GN2BL1 (By similarity).DOMAIN: SwissProt: Q02156 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.PTM: Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-PKCε (Ser729) Antibody | 06-821 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Rabbit View All»
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