货号：06-427

品牌：Merck Millipore

规格：100UL

目录价：￥6588.00

市场价格：￥5599.80

会员价格：￥5270.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphotyrosine Antibody | 06-427 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: The immunoreactivity of the antibody is totally inhibited by the use of 100 mM phenyl phosphate, a phosphotyrosine analog. The phosphotyrosine antibody is purified by immunoaffinity chromatography using either a dual phospho-peptide gel or a BSA-phosphotyrosine gel. All of the phosphotyrosine immunoreactivity present in the antisera is immunoadsorbed whether the antibody is purified by either gel indicating that the antibody is not sequence-specific but specific for phosphotyrosine residues. View All» Molecular Weight: Varies View All» Immunogen: In order to produce broad spectrum polyclonal phosphotyrosine antibodies, rabbits were immunized with three phosphorylated immunogens: (1) phosphotyrosine covalently linked to KLH; (2) the c-Src carboxyl terminal regulatory phosphopeptide (TS-T-E-P-Q-pY-Q-P-G-E-N-L; Catalog # 12-218) covalently linked to KLH, and; (3) a phosphopeptide associated with high tyrosine kinase activity in human lymphocytes (R-R-L-I-E-D-A-E-pY-A-A-R-G; Catalog # 12-217) covalently linked to KLH. Both of the phosphopeptide haptens serve as strong substrates for tyrosine phosphatases and are part of the two colorimetric tyrosine phosphatase kits provided by Upstate, Inc. (Catalog # 17-125, 17-126). View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Background Information: The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function. View All» Species Reactivity:

• Human

• Rat

• Mouse
  View All» Species Reactivity Note: Human, mouse and rat. Other species cross-reactivity is unknown. View All» Application Notes: Immunoprecipitation: 4 μg of a previous lot immunoprecipitated proteins containing phosphotyrosine residues from a human A431 RIPA lysate. View All» Control: Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Evaluated by western blot on EGF-stimulated human A431 carcinoma cells.Western Blot Analysis: 0.5-2 μg/mL of this antibody detected proteins containing phosphotyrosine residues in RIPA lysates from EGF-stimulated human A431 carcinoma cells. View All» Purification Method: Protein A Purified followed by immunoaffinity View All» Presentation: Purified rabbit polyclonal in storage buffer containing 0.2 M Tris-glycine, Ph 7.2, 0.15 M NaCl, 1 mg/mL of BSA containing 0.05% sodium azide. Frozen at -20°C. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Immunoprecipitation

  • Western Blotting
    View All» Brand Family: Upstate View All» Product Name: Anti-Phosphotyrosine Antibody | 06-427 View All» Concentration: 181.81 µg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Rabbit View All»
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