货号:06-262
品牌:Merck Millipore
规格:EA
目录价:¥3550.00
市场价格:¥3017.50
会员价格:¥2840.00
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Description:
Anti-GAL4 (DNA binding domain) Antibody | 06-262
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Upstate (Millipore)
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Specificity:
Recognizes the DNA-binding domain of GAL4.
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Molecular Weight:
106 kDa
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Immunogen:
The GAL4 amino terminal fragment of yeast, containing only the DNA-binding domain (1-147) expressed in E. coli
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Isotype:
IgG
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Background Information:
The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (amino acids 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells.
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Species Reactivity:
Yeast (S. cerevisiae)
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Control:
Liver tissue
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Quality Assurance:
routinely evaluated by immunoblot on a lysate prepared from insect cells infected with a baculovirus expressing GAL4 or in a yeast cell extract
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Purification Method:
Ammonium sulfate precipitation
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Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
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Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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UniProt Number:
P04386
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Entrez Gene Number:
NP_015076
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Immunoprecipitation
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Western Blotting
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Brand Family:
Upstate
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Product Name:
Anti-GAL4 (DNA binding domain) Antibody | 06-262
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Concentration:
Variable
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Antibody Type:
Polyclonal Antibody
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Qty/Pk:
250 μL
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Format:
Purified
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Host:
Rabbit
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