货号：05-949

品牌：Merck Millipore

规格：200Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Histidine Tagged Antibody, clone HIS.H8 | 05-949 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag. View All» Immunogen: 6x His synthetic peptide. View All» Clone: HIS.H8 View All» Isotype: IgG2b View All» Background Information: His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems. View All» Species Reactivity: All View All» Species Reactivity Note: All Species. View All» Application Notes: Immunocytochemistry: 2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells.Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins. View All» Control: RIPA lysates of transfected HeLa cells. View All» Quality Assurance: Routinely evaluated by western blot and immunocytochemistry on transfected HeLa cells.Western Blot Analysis: 0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells. View All» Purification Method: Purified View All» Presentation: Purified mouse monoclonal IgG2b in buffer containing PBS, pH 7.4 with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunocytochemistry

• Immunoprecipitation

• Western Blotting
  View All» Brand Family: Upstate View All» Product Name: Anti-Histidine Tagged Antibody, clone HIS.H8 | 05-949 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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