货号：05-825R

品牌：Merck Millipore

规格：100UL

目录价：￥3433.00

市场价格：￥2918.05

会员价格：￥2746.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PDGFR-β Antibody, clone 4A56 | 05-825R View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes PDGFR-β at the protein kinase domain. View All» Molecular Weight: ~ 190 kDa observed View All» Epitope: Protein kinase domain View All» Immunogen: KLH-conjugated linear peptide corresponding to human PDGFR-β at the protein kinase domain. View All» Isotype: IgG View All» Background Information: PDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and itsautophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Demonstrated to react with mouse. Predicted to react with human based on 100% sequence homology. View All» Application Notes: Immunoprecipitation Analysis: 0.4 µg from a previous lot immunoprecipitated PDGFR-β from BALB/3T3/A31 cells. View All» Control: NIH/3T3 cell lysate View All» Quality Assurance: Evaluated by Western Blot in NIH/3T3 cell lysate.Western Blot Analysis: 1:4,000 dilution of this antibody detected PDGFR-β on 10 µg of NIH/3T3 cell lysate. View All» Purification Method: Protein A View All» Presentation: Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P09619 View All» Entrez Gene Number: NP_002600 View All» Gene Symbol:
  • Becaplermin

  • PDGF-2

  • PDGF2

  • SIS

  • SSV

  • becaplermin

  • c-sis

  • PDGFRβ

  • PDGFR-β
    View All» Alternate Names:
    • PDGF subunit B

    • PDGF, B chain

    • Platelet-derived growth factor B chain

    • Platelet-derived growth factor beta polypeptide

    • Platelet-derived growth factor, beta polypeptide (oncogene SIS)

    • platelet-derived growth factor 2

    • platelet-derived growth factor beta

    • platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)

    • platelet-derived growth factor, B chain
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation
        View All» Entrez Gene Summary: This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.SUBUNIT STRUCTURE: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly. Interacts with GRB10. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain. View All» Product Name: Anti-PDGFR-β, clone 4A56 View All» Concentration: 0.02 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://16ao.com/thread-134072.htm
        © Merck Millipore代理05-825R Anti-PDGFR-ß, clone 4A56;store at -20℃，产品报价联系微信jinshanbio