货号：05-817R

品牌：Merck Millipore

规格：

目录价：￥4633.00

市场价格：￥3938.05

会员价格：￥3706.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant | 05-817R View All» Replaces: 05-817 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes Histone H3 phosphorylated at Serine 10. View All» Molecular Weight: 17 kDa View All» Immunogen: KLH-conjugated, synthetic peptide containing the sequence RK[pS]TG, in which pS corresponds to phospho-serine at position 10 of human histone H3 View All» Modifications: Phosphorylation View All» Clone: clone 63-1C-8 View All» Background Information: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine View All» Species Reactivity: Human View All» Species Reactivity Note: Broad species cross-reactivity is expected View All» Application Notes: Beadlyte® Histone-Peptide Specificity Assay: A 1:5,000 dilution of a previous lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. (Figure B). Only the peptide containing phospho-serine 10 was detected.Flow CytometryJurkat cells, untreated (red) or treated with calyculin A (blue), were detected with 1:100 anti-phospho-Histone H3 (Ser10), clone 63-1C-8. View All» Control: UV-treated 293 cell extracts, UV- or colcemid treated HeLa cell extracts, breast cancer tissue, HEPG2 cell extracts View All» Quality Assurance: Routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells.Western Blot Analysis: A 1:1,000 dilution of a recent lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells treated with colcemid View All» Purification Method: Protein A purfied View All» Presentation: Protein A purified recombinant antibody supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide, 40% glycerol. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. View All» UniProt Number: Q16695 View All» Entrez Gene Number: NM_003493 View All» Gene Symbol:

• HIST3H3

• H3FT

• MGC126886

• H3t

• MGC126888

• H3T

• H3/g

• H3.4

• H3/t
  View All» Alternate Names:
  • H3S10P

  • Histone H3 (phospho S10)
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Multiplexing

    • ELISA
      View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. View All» UniProt Summary: FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Expressed in testicular cells.DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant | 05-817R View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Purified View All» Host: Rabbit View All»
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