货号：05-669

品牌：Merck Millipore

规格：100Ug

目录价：￥5569.00

市场价格：￥4733.65

会员价格：￥4455.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6 | 05-669 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes phosphorylated Akt1/PKBα at Ser473, Mr 60 kDa. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473. View All» Molecular Weight: 60 kDa View All» Immunogen: KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473. View All» Modifications: Phosphorylation View All» Clone: 11E6 View All» Isotype: IgG1κ View All» Background Information: The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases. View All» Species Reactivity:

• Mouse

• Rat

• Human

• Canine
  View All» Species Reactivity Note: Human, mouse, canine and rat. View All» Application Notes: ELISA: A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA. View All» Control: PDGF treated NIH-3T3 cell lysates. View All» Quality Assurance: Evaluated by western blot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF.Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes. View All» Purification Method: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography View All» Presentation: Purified mouse monoclonal IgG1κ lyophilized from 1 mL 2X PBS, PEG, sucrose, and 0.09% sodium azide. Reconstitute with 1 mL H₂O (15 min. at RT). Concentration after reconstitution: 100 µg/mL View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Reconstitute prior to use. Reconstitute with 1 mL H₂O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P31749 View All» Entrez Gene Number: NM_005163 View All» Gene Symbol:
  • AKT1

  • RAC

  • PRKBA

  • MGC99656

  • RAC-ALPHA

  • RAC-PK-alpha

  • C-AKT

  • PKB

  • AKT
    View All» Alternate Names:
    • Protein kinase B

    • RAC-alpha serine/threonine-protein kinase

    • murine thymoma viral (v-akt) oncogene homolog-1

    • rac protein kinase alpha

    • v-akt murine thymoma viral oncogene homolog 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Western Blotting
        View All» Entrez Gene Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.SIZE: 480 amino acids; 55686 Da SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).TISSUE SPECIFICITY: In all human cell types so far analyzed.DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6 | 05-669 View All» Concentration: 100 μg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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