货号：05-633

品牌：Merck Millipore

规格：1EA

目录价：￥4570.85

市场价格：￥3885.22

会员价格：￥3656.68

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-BrdU Antibody, clone BU-1 | 05-633 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: 5-Bromo-2-deoxyuridine View All» Molecular Weight: none given View All» Immunogen: 5-iodouridine:ovalbumin conjugate View All» Isotype: IgG2a View All» Background Information: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. View All» Species Reactivity: All View All» Quality Assurance: A representative lot was evaluated by immunocytochemistry on HeLa cells. A 1:2-1:4 dilution from a representative lot showed positive immunostaining for Anti-BrdU in HeLa cells fixed with 95% ethanol, 5% glacial acetic acid. This lot was tested using the DNA Replication Assay Kit (Catalog # 17-316). Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required. View All» Purification Method: Concentrated culture supernatant View All» Presentation: 4 vials, each vial containing mouse culture supernatant lyophilized from 2.5 mL. The supernatant contains mycoplasma, which contribute enzymes that facilitate antibody binding. Lyophilized powder. View All» Storage Conditions: 2 years at -20°C View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunocytochemistry View All» Brand Family: Upstate View All» Product Name: Anti-BrdU Antibody, clone BU-1 | 05-633 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 4 vials View All» Format: Culture Supernatant View All» Host: Mouse View All»