货号：05-481

品牌：Merck Millipore

规格：100Ug

目录价：￥5513.00

市场价格：￥4686.05

会员价格：￥4410.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-MAP Kinase1/2 (Erk 1/2) Antibody, clone 12D4 | 05-481 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2. View All» Molecular Weight: 42-44 kDa View All» Immunogen: KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4 View All» Modifications: Phosphorylation View All» Clone: clone 12D4 View All» Isotype: IgG1κ View All» Background Information: The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand. Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Avian

• Canine
  View All» Application Notes: Immunoprecipitation: Use 1-10 μg per immuno-precipitation. View All» Control: Included Positive Control Lysate: Catalog # 12-302, EGF-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg (20 μL) of reduced lysate per lane for minigels. View All» Quality Assurance: Evaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells. View All» Purification Method: Thiophilic adsoption and size exclusion chromatography View All» Presentation: Purified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» UniProt Number: P27361 View All» Entrez Gene Number: NM_002745, NM_002746 View All» Gene Symbol:
  • MAPK3

  • MGC20180

  • ERT2

  • HUMKER1A

  • p44-MAPK

  • PRKM3

  • ERK1

  • p44erk1

  • p44mapk

  • P44MAPK

  • HS44KDAP

  • p44-ERK1

  • P44ERK1

  • ERK-1
    View All» Alternate Names:
    • Extracellular signal-regulated kinase

    • Insulin-stimulated MAP2 kinase

    • MAP kinase 1

    • MAPK 1

    • Microtubule-associated protein 2 kinase

    • extracellular signal-related kinase 1

    • mitogen-activated protein kinase 3
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • ELISA

      • Immunocytochemistry
        View All» Entrez Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. ... View All» UniProt Summary: FUNCTION: SwissProt: P27361 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).COFACTOR: Magnesium (By similarity).SIZE: 379 amino acids; 43136 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4.DOMAIN: SwissProt: P27361 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... View All» Brand Family: Upstate View All» Product Name: Anti-phospho-MAP Kinase1/2 (Erk 1/2) Antibody, clone 12D4 | 05-481 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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