货号：05-363

品牌：Merck Millipore

规格：200Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cyclin E Antibody, clone HE12 | 05-363 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: human p52cyclin E View All» Molecular Weight: 52 kDa View All» Immunogen: Recombinant human cyclin E View All» Isotype: IgG View All» Background Information: Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers. View All» Species Reactivity: Human View All» Control: A431 cell lysate View All» Quality Assurance: routinely evaluated in immunoblot on human A431 lysate View All» Purification Method: Protein G chromatography View All» Presentation: 0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide View All» Storage Conditions: 2 years at -20°C or 6 months at 4°C View All» UniProt Number: P24864 View All» Entrez Gene Number: NM_001238, NM_057182 View All» Gene Symbol:

• CCNE1

• CCNE
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Immunoprecipitation

  • Western Blotting

  • Immunocytochemistry
    View All» Entrez Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. View All» UniProt Summary: FUNCTION: SwissProt: P24864 # Essential for the control of the cell cycle at the G1/S (start) transition.SIZE: 410 amino acids; 47077 Da SUBUNIT: Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Interacts with retinoblastoma binding protein 3 and retinoblastoma-like protein 1. Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1.SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.PTM: Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY: SwissProt: P24864 ## Belongs to the cyclin family. Cyclin E subfamily. View All» Brand Family: Upstate View All» Product Name: Anti-Cyclin E Antibody, clone HE12 | 05-363 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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