货号：05-235

品牌：Merck Millipore

规格：200Ug

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-EF1α Antibody, clone CBP-KK1 | 05-235 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa. Occasionally, a 38 kDa protein is also detected in A431 cell lysate. View All» Molecular Weight: 53 kDa View All» Immunogen: Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography. Clone CBP-KK1. View All» Clone: CBP-KK1 View All» Isotype: IgG1κ View All» Background Information: Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The alpha 1 isoform is expressed in brain, placenta, lung, liver, kidney, and pancreas. The other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. The alpha 1 isoform is identified as an autoantigen in 66% of patients with Felty syndrome. The gene for the alpha 1 isoform has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. The gene for the alpha 2 may be critical in the development of ovarian cancer. View All» Species Reactivity:

• Bacteria

• Mammals

• Yeast (S. cerevisiae)
  View All» Species Reactivity Note: Trypanosomes, yeast, mammals View All» Application Notes: Immunoprecipitation: 4 µg of a previous lot immunoprecipitated EF1α from an A431 RIPA cell lysate. View All» Control: Jurkat cell lysate.Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol per 100 µL of lysate and boil for 5 minutes to reduce the preparation. Use 20 µg per lane for minigels. View All» Quality Assurance: Routinely evaluated on RIPA lysates from non-stimulated A431 cells, human Jurkat or mouse 3T3/A31.Western Blot Analysis: 0.5-2 µg/mL of this lot detected EF1α in RIPA lysates from non-stimulated A431 cells. Previous lots detected EF1α in human Jurkat and mouse 3T3/A31 RIPA cell lysates. View All» Purification Method: Protein G chromatography View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P68104 View All» Entrez Gene Number: NM_006620 View All» Gene Symbol:
  • EEF1A1

  • eEF1A-1

  • FLJ25721

  • EF-1-alpha-1

  • EEF-1

  • HNGC:16303

  • ALPHA

  • CCS-3

  • PTI1

  • MGC131894

  • EEF1A

  • CCS3

  • MGC102687

  • EF-Tu

  • MGC16224

  • GRAF-1EF

  • LENG7

  • EF1A
    View All» Alternate Names:
    • CTCL tumor antigen

    • EF1a-like protein

    • Elongation factor 1 A-1

    • Elongation factor Tu

    • Leukocyte receptor cluster member 7

    • cervical cancer suppressor 3

    • elongation factor 1 alpha subunit

    • elongation factor 1-alpha

    • eukaryotic translation elongation factor 1 alpha 1

    • eukaryotic translation elongation factor 1 alpha 1-like 14

    • glucocorticoid receptor AF-1 specific elongation factor

    • leukocyte receptor cluster (LRC) member 7

    • prostate tumor-inducing protein 1

    • translation elongation factor 1 alpha 1-like 14
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. View All» UniProt Summary: FUNCTION: SwissProt: P68104 # This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.SIZE: 462 amino acids; 50141 Da SUBUNIT: Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with XPO5.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.SIMILARITY: SwissProt: P68104 ## Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. View All» Brand Family: Upstate View All» Product Name: Anti-EF1α Antibody, clone CBP-KK1 | 05-235 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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