货号：05-163

品牌：Merck Millipore

规格：50Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PLCγ-1 Antibody | 05-163 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells1 and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4. View All» Specificity: This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992). View All» Molecular Weight: 135 kDa View All» Immunogen: Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain. View All» Clone: Clonal blend B-2-5, B-6-4, B-20-3, D-7-3, E-9-4. View All» Isotype: mixed IgGs View All» Background Information: Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Rabbit

• Bovine
  View All» Species Reactivity Note: Bovine, rabbit, rat, mouse and human. View All» Application Notes: Immunoprecipitation: 4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.Immunocytochemistry: 5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells. View All» Control: A431 cell lysate, HeLa cell lysateIncluded Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.Western Blot Analysis: 0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations. View All» Purification Method: Protein G chromatography View All» Presentation: Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as ascites. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4. View All» Storage Conditions: Stable for 1 year at -20ºC from date of shipment. View All» UniProt Number: P19174 View All» Entrez Gene Number: NM_002660.2 View All» Gene Symbol:
  • PLCG1

  • PLC1

  • PLCgamma1

  • PLC148

  • PLC-II

  • PLC-148

  • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate

  • PLC-gamma-1

  • inositoltrisphosphohydrolase
    View All» Alternate Names:
    • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1

    • Phosphoinositide phospholipase C

    • Phospholipase C-gamma-1

    • monophosphatidylinositol phosphodiesterase

    • phosphatidylinositol phospholipase C

    • phosphoinositidase C

    • phospholipase C gamma 1

    • phospholipase C, gamma 1

    • phospholipase C, gamma 1 (formerly subtype 148)

    • phospholipase C-148

    • triphosphoinositide phosphodiesterase
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P19174 # PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.COFACTOR: Calcium.SIZE: 1290 amino acids; 148532 Da SUBUNIT: Interacts with CENTG1 via its SH3 domain (By similarity). Interacts with phosphorylated LAT upon TCR activation. Interacts with the Pro-rich domain of TNK1 via its SH3 domain. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor- dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts via its SH3 domain with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1 (By similarity).PTM: The receptor-mediated activation of PLC-gamma-1 and PLC- gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. & Ubiquitinated by CBLB in activated T-cells (By similarity).SIMILARITY: SwissProt: P19174 ## Contains 1 C2 domain. & Contains 1 EF-hand domain. & Contains 2 PH domains. & Contains 1 PI-PLC X-box domain. & Contains 1 PI-PLC Y-box domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. ... View All» Brand Family: Upstate View All» Product Name: Anti-PLCγ-1 Antibody | 05-163 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Purified View All» Host: Mouse View All»
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