货号:MCD8MAG48K-PX15
品牌:Merck Millipore
规格:
目录价:¥36874.00
市场价格:¥31342.90
会员价格:¥29499.20
金山科研平台,产品价格货期咨询微信:jinshanbio
Description:
MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel Premimxed 15 Plex, MCD8MAG48K-PX15
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Trade Name:
MILLIPLEX
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Qty/Pk:
96-well plate
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Product Overview:
The MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel enables you to focus on the therapeutic potential of Mouse CD8+ T Cell analytes. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel is the most versatile system available for Mouse CD8+ T Cell research. • MILLIPLEX MAP offers you the ability to:• Select a 15-plex premixed option or• Choose any combination of analytes from our panel of 15 analytes to design a custom kit that better meets your needs.• A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.
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Background Information:
CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.
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Key Applications:
Multiplexing
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Application Notes:
• This is a 2-hr or overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.• This kit may be used for the analysis of all or any combination of the CD8+ T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant
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Species Reactivity:
Mouse
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Linearity of Dilution:
-
%
-
%
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Sensitivity:
Refer to kit protocol for sensitivities of individual biomarkers.
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Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
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Cross Reactivity:
There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol)..
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Product Name:
MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel Premimxed 15 Plex, MCD8MAG48K-PX15
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Standard Curve Range:
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sCD137 IFNγ, IL-2, MIP-1ß, RANTES: 4.9 – 20,000 pg/mL
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sFas, sFas Ligand, GM-CSF: 24.4 – 100,000 pg/mL
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Granzyme B, IL-4, TNFα: 2.4 – 10,000 pg/mL
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IL-5: 3.7 – 15,000 pg/mL
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IL-6: 12.2 – 50,000 pg/mL
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IL-10: 6.1 – 25,000 pg/mL
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IL-13: 48.8 – 200,000 pg/mL
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Analytes Available:
GM-CSF, Granzyme B, IFNγ, IL-10, IL-13, IL-2, IL-4, IL-5, IL-6, MIP-1β, RANTES, TNF-α, sCD137, sFAS ligand, sFas
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Configuration:
Premixed
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Panel Type:
MAGNETIC Cytokines/Chemokines
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Precision, %:
-
Inter-assay:
-
Intra-assay:
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Detection Methods:
Luminex xMAP
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