货号：MABT144

品牌：Merck Millipore

规格：

目录价：￥4259.00

市场价格：￥3620.15

会员价格：￥3407.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Alpha-Actinin 4 Antibody, clone EPR2533(2), Rabbit | MABT144 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Alpha-Actinin 4 at the N-terminus. View All» Molecular Weight: ~100 kDa observed View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to human Alpha-Actinin 4 at the N-terminus. View All» Clone: EPR2533(2) View All» Isotype: IgG View All» Background Information: Alpha-actinin is a ubiquitously expressed cytoskeletal protein found in numerous actin structures. It consists of an N-terminal actin-binding domain, a central rod domain, and a C-terminal domain; the protein functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinin 4 is a non-muscle isoform version of alpha-actinin that is concentrated in the cytoplasm (2). It plays a role in cell motility, invasion, and metastasis of cancer cells (3). Alpha-actinin 4 is commonly expressed in filopodia, lamellipodia, stress fibers, and sites of adhesion (4). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot detected Alpha-Actinin 4 in HeLa cells.Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected Alpha-Actinin 4 in human kidney tissue.Immunoprecipitation Analysis: A 1:10-100 dilution from a representative lot was used in IP. View All» Control: Human fetal skeletal muscle tissue lysate View All» Quality Assurance: Evaluated by Western Blot in human fetal skeletal muscle tissue lysate, A431, MCF-7 and HeLa cell lysates.Western Blot Analysis: A 1:1,000 to 1:10,000 dilution of this antibody detected Alpha-Actinin 4 in human fetal skeletal muscle tissue lysate, A431, MCF-7 and HeLa cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below-20°C may cause glycerol containing Solutions to become frozen during storage. View All» UniProt Number: O43707 View All» Gene Symbol:
  • FSGS

  • FSGS1

  • DKFZp686K23158
    View All» Alternate Names:
    • actinin, alpha 4

    • Non-muscle alpha-actinin 4

    • F-actin cross-linking protein

    • ACTININ-4

    • alpha-actinin-4

    • actinin alpha4 isoform
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation
        View All» UniProt Summary: FUNCTION: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.SUBUNIT STRUCTURE: Homodimer; antiparallel By similarity. Binds TRIM3 at the N-terminus By similarity. Identified in a complex with CASK, IQGAP1, MAGI2, NPHS1, SPTAN1 and SPTBN1 By similarity. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with BAIAP1 and PDLIM2. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF7, oral floor cancer IMC2, and bladder cancer KU7). TISSUE SPECIFICITY: Widely expressed.INVOLVEMENT IN DISEASE: Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. SEQUENCE SIMILARITIES: Belongs to the alpha-actinin family.Contains 1 actin-binding domain.Contains 2 CH (calponin-homology) domains.Contains 2 EF-hand domains.Contains 4 spectrin repeats.SEQUENCE CAUTION: The sequence AAC17470.1 differs from that shown. Reason: Frameshift at positions 19, 26, 124, 130, 589, 594, 787 and 806. The sequence BAA24447.1 differs from that shown. Reason: Erroneous initiation. View All» Product Name: Anti-Alpha-Actinin 4, clone EPR2533(2), Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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