货号：MABS174

品牌：Merck Millipore

规格：

目录价：￥3475.00

市场价格：￥2953.75

会员价格：￥2780.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ASIP Antibody, clone EP310Y, Rabbit | MABS174 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes ASIP near the C-terminus and is predicted to detect isoforms 2-7 based on sequence analysis. View All» Molecular Weight: ~150 kDa observed View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to human ASIP near the C-terminus. View All» Clone: EP310Y View All» Isotype: IgG View All» Background Information: The agouti signaling protein (ASIP) is an adaptor protein involved in asymmetrical cell division and cell polarization processes. ASIP seems to play a central role in the formation of epithelial tight junctions. ASIP may affect the quality of hair pigmentation, and act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone (2-3). ASIP may also play a role in neuroendocrine aspects of melanocortin action, and have a functional role in regulating lipid metabolism in adipocytes (4-5). View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Flow Cytometry Analysis: 1:20 dilution from a representative lot was used in FC. View All» Control: LNCap, HeLa, A431, and MCF-7 cell lysates View All» Quality Assurance: Evaluated by Western Blot in LNCap, HeLa, A431, and MCF-7 cell lysates.Western Blot Analysis: 1:500 dilution of this antibody detected ASIP in LNCap, HeLa, A431, and MCF-7 cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot intomicrocentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q8TEW0 View All» Entrez Gene Number: Q8TEW0 View All» Gene Symbol:

• PAR3

• ASIP

• Bazooka

• Baz

• PARD3A

• PAR3-alpha

• PARD-3

• SE2-5T2

• PAR3alpha

• OTTHUMP00000019427

• SE2-5L16

• FLJ21015

• SE2-5LT1

• OTTHUMP00000019434

• PAR-3

• PAR3A
  View All» Alternate Names:
  • par-3 partitioning defective 3 homolog (C. elegans)

  • Atypical PKC isotype-specific-interacting protein

  • CTCL tumor antigen se2-5

  • par-3 (partitioning defective 3, C.elegans) homolog

  • atypical PKC isotype-specific interacting protein

  • partitioning defective 3 homolog2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Flow Cytometry
      View All» UniProt Summary: FUNCTION: Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions By similarity. Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons. SUBUNIT STRUCTURE: Interacts (via PDZ 1 domain) with F11R/JAM1, PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. Interacts with PRCKI and CDH5. Interacts (via PDZ 3 domain) with PTEN (via C-terminus) By similarity. Part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3. Interacts with LIMK2, AURKA and AURKB. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. SUBCELLULAR LOCATION: Endomembrane system. Cell junction. Cell junction › tight junction. Cell membrane. Cytoplasm › cell cortex By similarity. Cytoplasm › cytoskeleton. Note: Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II By similarity. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.TISSUE SPECIFICITY: Widely expressed. DOMAIN: Contains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization By similarity.The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids By similarity.PTM: Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2. Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.MISCELLANEOUS: Antibodies against PARD3 are present in sera from patients with cutaneous T-cell lymphomas.SEQUENCE SIMILARITIES: Belongs to the PAR3 family.Contains 3 PDZ (DHR) domains.SEQUENCE CAUTION: The sequence AAG33676.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence BAA91366.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAB55330.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. View All» Product Name: Anti-ASIP, clone EP310Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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