货号：MABS170

品牌：Merck Millipore

规格：

目录价：￥18096.00

市场价格：￥15381.60

会员价格：￥14476.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ERK5 Antibody, clone EP791Y, Rabbit | MABS170 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Specificity: This antibody recognizes ERK5 at the C-terminus. View All» Molecular Weight: ~115 kDa observed View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to human ERK5 at the C-terminus. View All» Clone: EP791Y View All» Isotype: IgG View All» Background Information: The extracellular signal-regulated kinase 5 (ERK5), also known as MAPK7 or big mitogen-activated protein kinase 1 (BMK1), is a member of the MAP kinase subfamily (1). ERK5 differs considerably from other MAPKs; it contains an unusually long carboxyl-terminal tail which might contribute to the regulation of its activity and/or localization. In response to extracellular signals, ERK5 translocates to the nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors (2). ERK5 also differs from other MAPKs in possessing a potent transcriptional activation domain which mediates protein-protein interactions with the myocyte enhancer factor 2 (MEF2) transcription factors (3). ERK5 is specifically activated by MAPK kinase 5 (MAP2K5/MEK5) and plays an important role in mammary epithelial proliferation, endothelial cell survival and normal embryonic development (4-5). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Mouse, and Rat. View All» Application Notes: Immunocytochemistry Analysis: A 1:100-1:250 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: A 1:50 dilution from a representative lot was used in IP.Flow Cytometry Analysis: A 1:30 dilution from a representative lot was used in FC. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: A 1:5,000 dilution of this antibody detected ERK5 in HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q13164 View All» Entrez Gene Number: Q13164 View All» Gene Symbol:
  • ERK5

  • BMK1

  • PRKM7

  • BMK-1

  • ERK-5

  • MAPK 7

  • EC 2.7.11.24

  • ERK4

  • EC 2.7.11
    View All» Alternate Names:
    • mitogen-activated protein kinase 7

    • Big MAP kinase 1

    • Extracellular signal-regulated kinase 5

    • BMK1 kinase

    • extracellular-signal-regulated kinase 5
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Flow Cytometry

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium By similarity.ENZYME REGULATION: Activated by tyrosine and threonine phosphorylation By similarity. Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF). SUBUNIT STRUCTURE: Interacts with MAP2K5. Forms oligomers By similarity. Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D By similarity. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Translocates to the nucleus upon activation. TISSUE SPECIFICITY: Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. DOMAIN: The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.PTM: Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme By similarity. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.SEQUENCE CAUTION: The sequence AAA81381.1 differs from that shown. Reason: Frameshift at positions 19 and 32. The sequence BAD92848.1 differs from that shown. Reason: Erroneous initiation. View All» Product Name: Anti-ERK5, clone EP791Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Host: Rabbit View All»
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