货号：MABS160

品牌：Merck Millipore

规格：

目录价：￥3393.00

市场价格：￥2884.05

会员价格：￥2714.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho EZH2 (Thr487) Antibody, clone EPR1410, Rabbit | MABS160 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Ezh2 phosphorylated at Thr487. View All» Molecular Weight: ~100 kDa observed View All» Epitope: Phosphorylated Thr487 View All» Immunogen: Phospho specific peptide corresponding to human Ezh2 phosphorylated at Thr487. View All» Modifications: Phosphorylation View All» Clone: EPR1410 View All» Isotype: IgG View All» Background Information: Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo (2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Mouse and Rat. View All» Application Notes: Immunoprecipitation Analysis: A 1:10 dilution from a representative lot was used in IP. Immunohistochemistry Analysis: A 1:100-1:250 dilution from a representative lot was used in IH. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: A 1:1,000-1:10,000 dilution of this antibody detected Ezh2 in HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q15910 View All» Entrez Gene Number: Q15910 View All» Gene Symbol:
  • ENX-1

  • KMT6

  • EZH1

  • KMT6A

  • EC 2.1.1.43

  • ENX1

  • MGC916

  • OTTHUMP00000213774

  • OTTHUMP00000213778

  • OTTHUMP00000213779

  • OTTHUMP00000213780

  • EC 2.1.1
    View All» Alternate Names:
    • enhancer of zeste homolog 2 (Drosophila)

    • Lysine N-methyltransferase 6

    • enhancer of zeste (Drosophila) homolog 2

    • enhancer of zeste 2

    • histone-lysine N-methyltransferase EZH2

    • Enhancer of zeste homolog 2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry

      • Immunoprecipitation
        View All» UniProt Summary: FUNCTION: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].SUBUNIT STRUCTURE: Binds ATRX via the SET domain Probable. Component of the PRC2/EED-EZH2 complex, which includes EED, EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. The minimum components required for methyltransferase activity of the PRC2/EED-EZH2 complex are EED, EZH2 and SUZ12. The PRC2 complex may also interact with DNMT1, DNMT3A, DNMT3B and PHF1 via the EZH2 subunit and with SIRT1 via the SUZ12 subunit. Interacts with HDAC1 and HDAC2. Interacts with PRAME. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICITY: Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. DEVELOPMENTAL STAGE: Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.INDUCTION: Expression is induced by E2F1, E2F2 and E2F3. Expression is reduced in cells subject to numerous types of stress including UV-, IR- and bleomycin-induced DNA damage and by activation of p53/TP53. PTM: Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. SEQUENCE SIMILARITIES: Belongs to the histone-lysine methyltransferase family. EZ subfamily.Contains 1 SET domain.CAUTION: Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2 View All» Product Name: Anti-phospho EZH2 (Thr487), clone EPR1410, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://16ao.com/thread-133157.htm
        © Merck Millipore代理MABS160 Anti-phospho Ezh2 (Thr487), clone EPR1410, Rabbit Monoclonal，产品报价联系微信jinshanbio