货号：MABS133

品牌：Merck Millipore

规格：

目录价：￥3574.00

市场价格：￥3037.90

会员价格：￥2859.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CHIP Antibody, clone 2F2.1 | MABS133 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~40 kDa observed. Uniprot describes two isoforms at ~35 kDa and ~27 kDa. Uniprot describes that this protein exists as a homodimer at ~80 kDa. Uniprot describes that this protein is phosphorylated upon DNA damage and is also ubiquinated. View All» Immunogen: GST-tagged recombinant protein corresponding to mouse CHIP. View All» Clone: 2F2.1 View All» Isotype: IgMκ View All» Background Information: Carboxyl-terminus of Hsc70 interacting protein (CHIP) is an E3 ubiquitin ligase. It has been detected in the heart, pancreas, brain, placenta, liver, lung, kidney, and skeletal muscle. CHIP is a multidomain protein containing an N-terminal TPR domain and a C-terminal U box domain both of which may interact with chaperones such as Hsc70 and Hsp70. CHIP is mainly involved with recognizing and marking misfolded proteins for degradation; however, CHIP is a unique E3 protein in requiring chaperone association with target proteins before marking them for ubiquitination. CHIP is therefore important for cell survival as it prevents the accumulation of non-functional proteins in the cell which could potentially produce cytotoxic effects. Previous studies have also suggested that CHIP may be involved in Toll-like receptor (4 and 6) signaling by activating downstream targets such as Src and PKCζ. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react in human. Predicted to react with mouse based on immunogen design. View All» Application Notes: Immunohistochemistry Analysis: A 1:50 dilution from a representatative lot detected CHIP in human placenta tissue. View All» Control: Human kidney tissue lysate View All» Quality Assurance: Evaluated by Western Blot in human kidney tissue lysate.Western Blot Analysis: 0.25 µg/mL of this antibody detected CHIP in 10 µg of human kidney tissue lysate. View All» Purification Method: Purifed by ion-exchange chromatography View All» Presentation: Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9UNE7 View All» Entrez Gene Number: NP_005852 View All» Gene Symbol:

• STUB1

• CHIP

• PP1131
  View All» Alternate Names:
  • E3 ubiquitin-protein ligase CHIP

  • Antigen NY-CO-7

  • CLL-associated antigen KW-8

  • Carboxy terminus of Hsp70-interacting protein

  • STIP1 homology and U box-containing protein 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry

    • Western Blotting
      View All» Entrez Gene Summary: STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation. View All» UniProt Summary: FUNCTION: E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation.PATHWAY: Protein modification; protein ubiquitination.SUBUNIT STRUCTURE: Homodimer. Interacts with BAG2, and with the E2 ubiquitin conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Interacts with the C-terminal domains of HSPA8 and HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with MKKS. Interacts with DYX1C1 and POLB. Interacts (via TPR repeats) with HSP90AA1. Interacts (via the U-box domain) with the UBE2V2-UBE2N heterodimer; the complex has a specific 'Lys-63'-linked polyubiquitination activity.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung.DOMAIN: The TPR domain is essential for ubiquitination mediated by UBE2D1.POST-TRANSLATIONAL MODIFICATION: Phosphorylated upon DNA damage, probably by ATM or ATR. Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2.MISCELLANEOUS: Antibodies against STUB1 are found in patients with chronic lymphocytic leukemia (CLL) and in colorectal cancer patients.SEQUENCE SIMILARITIES: Contains 3 TPR repeats. Contains 1 U-box domain. View All» Product Name: Anti-CHIP, clone 2F2.1 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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