货号：MABF29

品牌：Merck Millipore

规格：100UL

目录价：￥4967.00

市场价格：￥4221.95

会员价格：￥3973.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-IL-16 Antibody, clone 14.1 | MABF29 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~20 kDa observed.Monomers of IL-16 migrate on SDS-PAGE in the range of 17–20 kDa (Zhang, Y., et al., 1998). Active IL-16 (clone 14.1) is detected at ~17 kDa in WB (Skundric, D., et al., 2005). View All» Epitope: Unknown View All» Immunogen: GST-tagged recombinant protein corresponding to the C-terminal region of secreted human IL-16. View All» Clone: 14.1 View All» Background Information: Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH to detect IL-16 in naive kidney tissue (Wang, S., et al., 2008).Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC to detect IL-16 in spinal cord cells (Skundric, D., et al., 2005).Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect IL-16 in human PBMCs (McFadden, C., et al., 2007).Neutralizing Activity Assay: 1–10 µg/mL of a previous lot was used by an independent laboratory at concentrations sufficient to neutralize bioactivity of IL-16 (Morgan, R., et al., 2007). View All» Control: Human serum View All» Quality Assurance: Evaluated by Western Blot in human serum.Western Blot Analysis: A 1:1,000 dilution of this antibody detected IL-16 in 10 µg of human serum. View All» Purification Method: Protein G Purified View All» Presentation: Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q14005 View All» Entrez Gene Number: NP_001165599 View All» Gene Symbol:

• IL-16

• PRIL16

• LCF

• IL16

• NIL16

• prIL-16
  View All» Alternate Names:
  • interleukin 16 (lymphocyte chemoattractant factor)

  • lymphocyte chemoattractant factor

  • pro-interleukin-16

  • neuronal interleukin 16

  • prointerleukin 16
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Neutralizing

    • Immunohistochemistry

    • Immunocytochemistry

    • Flow Cytometry
      View All» Entrez Gene Summary: The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane. Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. SUBUNIT STRUCTURE: Homotetramer Probable; according (Ref.19) the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C By similarity. Isoform 3 interacts with HTLV-1 tax. SUBCELLULAR LOCATION: Interleukin-16: Secreted Isoform 1: Cytoplasm (By similarity). Isoform 3: Cytoplasm. Nucleus TISSUE SPECIFICITY: Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.INDUCTION: Isoform 3 is down-regulated in T-cells after TCR activation. PTM: Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. SEQUENCE SIMILARITIES: Contains 4 PDZ (DHR) domains. View All» Product Name: Anti-IL-16, clone 14.1 View All» Concentration: 0.25 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Mouse View All»
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