货号：MABE61

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-macro-H2A1.2 Antibody, clone 14G7 | MABE61 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~39 kDa observed View All» Immunogen: GST-tagged recombinant protein corresponding to human macro-H2A1.2. View All» Clone: 14G7 View All» Background Information: Histone MacroH2A (mH2A), also known as H2AFY, is a vertebrate-specific histone variant associated with condensed, inactive heterochromatin. This histone variant consists of a histone H2A-like domain fused to a large non-histone region. mH2A has been shown to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. The non-histone region of mH2A is directly involved in the repression of transcription, histone acetylation and blocking SWI/SNF and ACF nucleosome remodeling. This variant histone is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Sporn, J.C., et al. (2009). Oncogene. 1–6.)Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Sporn, J.C., et al. (2009). Oncogene. 1–6.) View All» Control: MCF-7 cell lysate View All» Quality Assurance: Evaluated by Western Blot in MCF-7 cell lysate.Western Blot Analysis: 0.25 µg/mL of this antibody detected macro-H2A1.2 on 10 µg of MCF-7 cell lysate. View All» Purification Method: Protein G View All» Presentation: Purified mouse monoclonal IgG2bλ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O75367 View All» Entrez Gene Number: NP_001035248 View All» Gene Symbol:

• H2A.y

• H2A/y

• H2AF12M

• H2AFJ

• MACROH2A1

• MACROH2A1.1

• mH2A1

• macroH2A1.2

• macro-H2A1.2
  View All» Alternate Names:
  • H2A histone family, member Y

  • Histone macroH2A1

  • Medulloblastoma antigen MU-MB-50.205

  • histone macroH2A1.1

  • histone macroH2A1.2

  • Core histone macro-H2A.1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry
      View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. TISSUE SPECIFICTY: Ubiquitous. PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.MISCELLANEOUS: The number of individuals with antibodies against this antigen is 2-fold higher in pediatric patients with cancer compared to healthy controls.SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.Contains 1 Macro domain. View All» Product Name: Anti-macro-H2A1.2, clone 14G7 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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