货号：MABE327

品牌：Merck Millipore

规格：100Ug

目录价：￥5974.00

市场价格：￥5077.90

会员价格：￥4779.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-p53 (pantropic) Antibody, clone DO-1 | MABE327 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of p53 (pantropic). View All» Molecular Weight: ~53 kDa observed. Uniprot describes 9 different isoforms due to alternative splicing and post-translational modification. This antibody detects isoform 1 (~38 kDa), isoform 2 (~39 kDa), and isoform 3 (~39 kDa). An uncharacterized band may be observed at ~43 kDa in some cell lysates. View All» Epitope: N-terminus View All» Immunogen: Recombinant protein corresponding to human p53 (pantropic). View All» Clone: DO-1 View All» Isotype: IgG2aκ View All» Background Information: Cellular tumor antigen p53 (p53) is a ubiquitous transcription factor that is most widely known for its function as a tumor suppressor, through its regulation of cell growth and survival. p53 is activated in response to cell stress such as DNA damage and induces either cell cycle arrest or apoptosis. It coordinates the expression of multiple genes including p21, GADD45, Bax, and Puma. p53 is regulated by multiple mechanisms: At the post-transcriptional level, the stability of p53 mRNAs is regulated by the binding of molecules such as HuR, Wig-1, RPL26, L26, and WRAP53. Post-translationally, p53 undergoes phosphorylation and acetylation which may also play a role in regulating its activity. It may also undergo ubiquitination via the Akt-MDM2 pathway resulting in degradation by the 20S proteosome. Mutations in p53 are prevalent in many types of cancers, underscoring the therapeutic relevance of this protein. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p53 (pantropic) in A431 cells.Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal adenocarcinoma tissue and in human prostate cancer tissue.Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal cancer cells.Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in radiolabelled HOS cell lysate (Vojtesek, B., et al. (1995). 71(6):1253-1256.).Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in primary breast carcinoma tissue (Beck, T., et al. (1995). Gynecol Oncol. 57(1):96-104.). View All» Control: A431 cell lysate View All» Quality Assurance: Evaluated by Western Blot in A431 cell lysate.Western Blot Analysis: 1 µg/mL of this antibody detected p53 (pantropic) in 10 µg of A431 cell lysate. This antibody detects isoforms 1, 2, and 3. View All» Purification Method: Protein G purified View All» Presentation: Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P04637 View All» Entrez Gene Number: NP_000537 View All» Gene Symbol:

• TP53

• P53
  View All» Alternate Names:
  • Cellular tumor antigen p53

  • Antigen NY-CO-13

  • Phosphoprotein p53

  • Tumor suppressor p53
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry

    • Immunofluorescence

    • Immunoprecipitation
      View All» Entrez Gene Summary: This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. View All» Product Name: Anti-p53 (pantropic), clone DO-1 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://16ao.com/thread-132972.htm
      © Merck Millipore代理MABE327 Anti-p53 (pantropic) Antibody, clone DO-1;store at +2℃ to +8℃，产品报价联系微信jinshanbio