货号：MABE308

品牌：Merck Millipore

规格：100UL

目录价：￥5513.00

市场价格：￥4686.05

会员价格：￥4410.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10 | MABE308 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~90 kDa observed View All» Immunogen: Recombinant protein corresponding to human ICBP90. View All» Clone: 1RC-1C10 View All» Isotype: IgG1 View All» Background Information: Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Western Blot Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in HeLa and MOLT-4 cell lysates (Hopfner, R., et al. (2001). 266(1-2)15-23.).Immunocytochemistry Analyisis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in ICC (Hopfner, R., et al. (2001). Gene. 266(1-2)15-23.).Immunohistochemistry Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in human appendix and human breast carcinoma tissues (Hopfner, R., et al. (2000). Cancer Res. 60(1):121-128.).Chromatin Immunoprecipitation Analyis: A representative lot from an independent laboratory immunoprecipitated ICB90/UHRF1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19). 8355-8365.) View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: A 1:5,000 dilution of this antibody detected ICBP90/UHRF1 in 10 µg of HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Mouse monoclonal IgG1 ascites with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q96T88 View All» Entrez Gene Number: NP_001041666 View All» Gene Symbol:

• UHRF1

• ICBP90

• NP95

• RNF106
  View All» Alternate Names:
  • E3 ubiquitin-protein ligase

  • inverted CCAAT box-binding protein

  • Nuclear protein 95

  • Nuclear zinc finger protein Np95

  • HuNp95

  • RING finger protein 106

  • Transcription factor ICBP90

  • Ubiquitin-like PHD and RING finger domain-containing protein 1

  • Ubiquitin-like-containing PHD

  • and RING finger domains protein 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Chromatin Immunoprecipitation (ChIP)

    • Immunocytochemistry

    • Immunohistochemistry
      View All» Entrez Gene Summary: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.PATHWAY: Protein modification; protein ubiquitination.SUBUNIT STRUCTURE: Interacts with histones H3, H1 and H2B. Interacts with HDAC1, but not with HDAC2. Interacts with UHRF1BP1. Binds methylated CpG containing oligonucleotides.SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.DEVELOPMENTAL STAGE. Expressed in fetal thymus, liver and kidney.INDUCTION: Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a p53/TP53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.DOMAIN:The RING finger is required for ubiquitin ligase activity. The YDG domain mediates the interaction with histone H3POST-TRANSLATIONAL MODIFICATION: Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity. Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.SEQUENCE SIMILARITIES: Contains 1 PHD-type zinc finger. Contains 2 RING-type zinc fingers. Contains 1 ubiquitin-like domain. Contains 1 YDG domain.SEQUENCE CAUTION: The sequence BAB15177.1 differs from that shown. Reason: Erroneous initiation. View All» Product Name: Anti-ICBP90/UHRF1, clone 1RC1C-10 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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