货号：MABE202

品牌：Merck Millipore

规格：EA

目录价：￥3430.00

市场价格：￥2915.50

会员价格：￥2744.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MEF2A Antibody, clone EP1706Y, Rabbit | MABE202 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of MEF2A. View All» Molecular Weight: ~66 kDa observed View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to amino acids located near the N-terminus of human MEF2A. View All» Clone: EP1706Y View All» Isotype: IgG View All» Background Information: MEF2A (myocyte specific enhancer factor 2) is a transcription factor protein that belongs to MEF2 family, a family of DNA binding regulatory proteins. MEF2 family members (MEF2A, 2B, 2C, and 2D) are highly expressed during dendritic maturation and synapse formation in the brain and are involved in the regulation of development and function of T-cell, muscle cells, and brain cells (1). Highly expressed during synaptogenesis, MEF2A binds to MEF2 element present in the regulatory regions in various muscle specific genes and activates transcription. MEF2A has been implicated in cardiac and skeletal muscle development (2). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Mouse, and Rat. View All» Application Notes: Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Flow Cytometry Analysis: 1:40 dilution from a representative lot was used in FC.Immunohistochemistry Analysis: 1:50-1:100 dilution from a representative lot detected MEF2A in human kidney tissue. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in fetal brain, human heart, and HeLa lysates.Western Blot Analysis: 1:10,000 dilution of this antibody detected MEF2A in fetal brain, human heart, and HeLa lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below-20°C may cause glycerol containing Solutions to become frozen during storage. View All» UniProt Number: Q02078 View All» Gene Symbol:
  • RSRFC9

  • RSRFC4

  • ADCAD1

  • MEF2
    View All» Alternate Names:
    • myocyte enhancer factor 2A

    • Serum response factor-like protein 1

    • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)

    • myocyte-specific enhancer factor 2A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Flow Cytometry

      • Immunohistochemistry
        View All» UniProt Summary: FUNCTION: Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. SUBUNIT STRUCTURE: Binds DNA as a homo- or heterodimer. Dimerizes with MEF2D. Interacts with HDAC7 By similarity. Interacts with PIAS1; the interaction enhances sumoylation. Interacts with HDAC4, HDAC9 and SLC2A4RG. Interacts (via the N-terminal) with MAPK7; the interaction results in the phosphorylation and transcriptional activity of MEF2A. SUBCELLULAR LOCATION: Nucleus TISSUE SPECIFICITY: Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined. PTM: Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis By similarity. Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation By similarity. Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. INVOLVEMENT IN DISEASE: Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].SEQUENCE SIMILARITIES: Belongs to the MEF2 family.Contains 1 MADS-box domain.Contains 1 Mef2-type DNA-binding domain. View All» Product Name: Anti-MEF2A, clone EP1706Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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