货号：MABE188

品牌：Merck Millipore

规格：

目录价：￥3522.00

市场价格：￥2993.70

会员价格：￥2817.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MCM7 Antibody, clone EP1974Y, Rabbit | MABE188 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the C-terminus of MCM7. View All» Molecular Weight: ~88 kDa observed View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to the C-terminus of human MCM7. View All» Clone: EP1974Y View All» Isotype: IgG View All» Background Information: The minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with Human, Mouse, and Rat. View All» Application Notes: Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: 1:40 dilution from a representative lot was used in IP.Flow Cytometry Analysis: 1:40 dilution from a representative lot was used in FC.Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected MCM7 in human lung tissue. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: 1:10,000 dilution of this antibody detected MCM7 in HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below-20°C may cause glycerol containing Solutions to become frozen during storage. View All» UniProt Number: P33993 View All» Gene Symbol:
  • CDC47

  • MCM2

  • P1.1-MCM3

  • CDC47 homolog

  • PNAS146

  • P85MCM

  • P1CDC47
    View All» Alternate Names:
    • minichromosome maintenance complex component 7

    • MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)

    • minichromosome maintenance deficient (S. cerevisiae) 7

    • minichromosome maintenance deficient 7

    • homolog of S. cerevisiae Cdc47

    • DNA replication licensing factor MCM7
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Flow Cytometry

      • Immunoprecipitation

      • Immunohistochemistry
        View All» UniProt Summary: FUNCTION: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.SUBUNIT STRUCTURE: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR-ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. SUBCELLULAR LOCATION: Nucleus By similarity. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. MISCELLANEOUS: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.SEQUENCE SIMILARITIES: Belongs to the MCM family.Contains 1 MCM domain. View All» Product Name: Anti-MCM7, clone EP1974Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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