货号：MABE15

品牌：Merck Millipore

规格：

目录价：￥4633.00

市场价格：￥3938.05

会员价格：￥3706.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Histone H2B Antibody, clone EP957Y, Rabbit | MABE15 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Histone H2B. View All» Molecular Weight: ~14 kDa observed View All» Immunogen: Synthetic peptide corresponding to human Histone H2B. View All» Clone: EP957Y View All» Isotype: IgG View All» Background Information: Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Proven to react with human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: A 1:250 – 1:500 dilution from a previous lot detected Histone H2B in human breast carcinoma.Immunoprecipitation Analysis: A 1:30 dilution from a previous lot detected Histone H2B in IP.Immunocytochemistry Analysis: A 1:50 – 1:100 dilution from a previous lot detected Histone H2B in HeLa cells. View All» Control: A431 cell lysate View All» Quality Assurance: Evaluated by Western Blot in A431 cell lysate.Western Blot Analysis: A 1:1,000 - 1:10,000 dilution of this antibody detected Histone H2B in A431 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q16778 View All» Entrez Gene Number: NP_001019770.1 View All» Gene Symbol:
  • H2B.1

  • H2B/q

  • H2BFQ

  • H2B

  • MGC119804

  • MGC129733

  • H2BGL105

  • GL105

  • H2BQ

  • MGC129734

  • MGC119802
    View All» Alternate Names:
    • histone cluster 2, H2be

    • histone 2, H2be

    • H2B histone family, member Q

    • Histone H2B.q

    • Histone H2B-GL105

    • histone H2B type 2-E
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)

      • Immunocytochemistry

      • Immunoprecipitation
        View All» UniProt Summary: FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus. Chromosome. PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. SEQUENCE SIMILARITIES: Belongs to the histone H2B family. View All» Product Name: Anti-Histone H2B, clone EP957Y, Rabbit Monoclonal Antibody (MABE15) View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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