货号：MABC135

品牌：Merck Millipore

规格：

目录价：￥4118.00

市场价格：￥3500.30

会员价格：￥3294.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ATG12 Antibody, clone EPR4800, Rabbit | MABC135 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes ATG12 at the C-terminus. View All» Molecular Weight: ~55 kDa observed. The observed band at ~55 kDa represents the ATG12-ATG5 conjugated form in endogenous lysates (Huang, J., et al. (2009) PNAS. 106(15):6226-6231). View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to human ATG12 at the C-terminus. View All» Clone: EPR4800 View All» Isotype: IgG View All» Background Information: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with, mouse and rat based on 100% sequence homology. View All» Application Notes: Immunocytochemistry Analysis: A 1:100-250 dilution from a representative lot was used in IC. Immunohistochemistry Analysis: A 1:100-250 dilution from a representative lot detected ATG12 in human breast carcinoma tissue. View All» Control: Fetal kidney tissue lysate, HepG2 and Raji cell lysates View All» Quality Assurance: Evaluated by Western Blot in fetal kidney tissue lysate, HepG2 and Raji cell lysates.Western Blot Analysis: A 1:1,000-10,000 dilution of this antibody detected ATG12 in Fetal kidney tissue lysate, HepG2 and Raji cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: O94817 View All» Gene Symbol:
  • APG4A

  • AUTL2

  • hAPG4A

  • OTTHUMP00000023825

  • EC 3.4.22.-

  • Autophagin-2
    View All» Alternate Names:
    • ATG4 autophagy related 4 homolog A (S. cerevisiae)

    • Autophagy-related cysteine endopeptidase 2

    • Autophagy-related protein 4 homolog A

    • AUT-like 2 cysteine endopeptidase

    • AUT-like 2, cysteine endopeptidase (S. cerevisiae)

    • APG4 autophagy 4 homolog A (S. cerevisiae)

    • autophagin 2

    • AUT-like 2, cysteine endopeptidase

    • autophagin-2

    • cysteine protease ATG4A

    • APG4 autophagy 4 homolog A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP.ENZYME REGULATION: Inhibited by N-ethylmaleimide.SUBCELLULAR LOCATION: Cytoplasm Probable. TISSUE SPECIFICITY: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. SEQUENCE SIMILARITIES: Belongs to the peptidase C54 family. View All» Product Name: Anti-ATG12, clone EPR4800, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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