货号：MAB88078

品牌：Merck Millipore

规格：

目录价：￥3552.00

市场价格：￥3019.20

会员价格：￥2841.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Heat Shock Factor I Antibody, clone 10H8 | MAB88078 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: MAB88078 recognizes a protein of 70-85 kDa (depending upon the souce and state of cells), identified as Heat Shock Factor 1 (HSF1). Reactivity with delection mutants suggests that the epitope of MAB88078 spans in the aa 378-395 of mouse HSF1. The synthesis of heat shock proteins is dependent on transcriptional and post-transcriptional control in eukaryotic cells. The regulation of heat shock gene transcription is mediate by the transcriptional activator, Heat Shock Factor (HSF), which binds to heat shock response elements (HSEs). At least three HSFs have been isolated from the human, mouse, chicken and tomato genomes and an additional factor, HSF4 has been identified in humans. HSFs purified from yeast, Drosophila, and human have different molecular weights and do not cross-react immunologically. These factors are activated by distinct stimuli. HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents whereas, the HSF2 is activated during differentiation. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA. The phosphorylation of HSF1 may be necessary for maximal transcription of heat shock genes. Cellular Localization: Cytoplasmic domain View All» Immunogen: Full length recombinanat mouse HSF1 protein (aa 1-503). View All» Clone: 10H8 View All» Isotype: IgG1 View All» Species Reactivity:

• Human

• Mouse

• Monkey
  View All» Application Notes: I mmunofluorescence (predominantly nuclear) Immunoprecipitation: 10 μL/mg cell lysate Western Blot: 1:200-1:400 (for 2hrs. at Room Temperature) Optimal working dilutions must be determined by end user. View All» Control: POSITIVE CONTROL: NIH-3T3, K562, HL-60, HEP-2, or HeLa cells View All» Presentation: Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide. View All» Storage Conditions: Maintain frozen at -20°C in undiluted aliquots for up to 12 months. View All» UniProt Number: Q00613 View All» Entrez Gene Number: NM_005526.2 View All» Gene Symbol:
  • HSF1

  • HSTF1
    View All» Alternate Names: HSFI View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Immunofluorescence

    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. View All» UniProt Summary: FUNCTION: SwissProt: Q00613 # DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.SIZE: 529 amino acids; 57260 Da SUBUNIT: DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a monomer. Interacts with SYMPK and CSTF2 in heat-stressed cells.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.SIMILARITY: SwissProt: Q00613 ## Belongs to the HSF family. View All» Brand Family: Chemicon View All» Product Name: Anti-Heat Shock Factor I Antibody, clone 10H8 | MAB88078 View All» Concentration: 0.2 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Purified View All» Host: Rat View All»
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