货号：05-1502

品牌：Merck Millipore

规格：100Ug

目录价：￥3999.00

市场价格：￥3399.15

会员价格：￥3199.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CENP-E Antibody, clone177 | 05-1502 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes CENP-E. View All» Molecular Weight: 312 kDa Calculated View All» Epitope: Unknown View All» Immunogen: Native protein corresponding to human CENP-E. View All» Clone: 177 View All» Isotype: IgG1κ View All» Background Information: Centromeric protein E (CENP-E) is a mitosis-specific kinesin belonging to the kinesin-7 family. CENP-E, along with cytoplasmic dyenin, is one of two main microtubule motors known to localize at kinetochores. During mitosis, CENP-E has an active role in the migration of chromosomes toward the metaphase plate, and is directly responsible for microtubule capture and stabilization by kinetochores. Reduction in CENP-E expression results in the high occurrence of whole chromosome missegregation, and its removal or inhibition leads to the presence of unattached chromosomes near the spindle pole as well as a significant reduction in the number of microtubules captured by kinetochores. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Immunohistochemistry Analysis: 1:100 dilution from a representative lot of this antibody detected CENP-E in kidney and malignant melanoma tissue.Western Blot Analysis: A representative lot of this antibody was used by an independent laboratory in WB. (Yen, T.J., et al. (1991). EMBO J. 10(5):1245–1254.) View All» Control: HeLa cells View All» Quality Assurance: Evaluated by Immunocytochemistry in HeLa cells.Immunocytochemistry Analysis: 1:500 dilution of this antibody detected CENP-E in HeLa cells. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q02224 View All» Entrez Gene Number: NP_001804 View All» Gene Symbol:

• CENP-E3

• CENP-E
  View All» Alternate Names:
  • centromere protein E, 312kDa

  • kinesin family member 10

  • centromere protein E (312kD)

  • Kinesin-related protein CENPE

  • Centromere autoantigen E (312kD)

  • Centromeric protein E

  • centromere protein E
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry

    • Western Blotting

    • Immunoprecipitation
      View All» Entrez Gene Summary: Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. SUBUNIT STRUCTURE: Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1.SUBCELLULAR LOCATION: Kinetochore. Cytoplasm › cytoskeleton › spindle. Centromere. Note: Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division. DOMAIN: The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part (By similarity).PTM: The C-teminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity). Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore. SEQUENCE SIMILARITIES: Belongs to the kinesin-like protein family.Contains 1 kinesin-motor domain.Contains 25 LRR (leucine-rich) repeats. View All» Product Name: Anti-CENP-E, clone177 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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