货号：05-1461

品牌：Merck Millipore

规格：

目录价：￥3697.00

市场价格：￥3142.45

会员价格：￥2957.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Src Family (WTAPE) Antibody, clone 2E8.2 | 05-1461 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes Src Family Members at the following amino acids:Src:424-435, Lyn:402-413, Fyn:425-436, Yes:431-442, Lck:399-411, Hck:394-405, Blk:394-405, and Fgr: 417-428. View All» Molecular Weight: ~60 kDa View All» Epitope: C-term WTAPE region View All» Immunogen: Shared Src family sequence found at the C-term. Amino acid sequence GAKFPIKWTAPE. View All» Clone: 2E8.2 View All» Isotype: IgG3κ View All» Background Information: pp60c-Src (Src) is the prototype non-receptor tyrosine kinase Src was the first proto-oncogenic protein discovered. The 9 members of the Src family include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk. They share common structural characteristics that include an N-terminal anchor sequence that allows the protein to the membrane followed by both an SH3 and an SH2 domain, a catalytic domain, an activation loop, and finally a C-term region that binds to the SH2 domain when phosphorylated (Tyr527 in Src that is phosphorylated by Csk). This binding causes Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target, and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype. Homology to Src in three regions is shared by a number of proteins, SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human and Rat tested. Expected to react with Mouse. View All» Control: A431 Cell Lysate View All» Quality Assurance: Routinely evaluated by Western BlotWestern Blot Analysis: This lot detected Src family members at a 2μg/mL dilution in EGF-treated A431 cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P). View All» Purification Method: Protein G purfied View All» Presentation: Protein G purified mouse monoclonal in PBS and 0.05% NaN3. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance View All» UniProt Number: P12931 View All» Entrez Gene Number: NP_005408 View All» Gene Symbol:
  • pp60c-src

  • p60-Src

  • c-Src
    View All» Alternate Names:
    • Integrin-linked kinase

    • 59 kDa serine/threonine-protein kinase
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Summary for proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] (accession no. NP_005408) (one example of Src family proteins containing the immunogen sequence: other members include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk).The gene for the proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: Summary for SRC_HUMAN (accession no. P12931) (one example of Src family proteins containing the immunogen sequence: other members include Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk).CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. SIMILARITY: Contains 1 protein kinase domain. SIMILARITY: Contains 1 SH2 domain. SIMILARITY: Contains 1 SH3 domain. View All» Brand Family: Upstate View All» Product Name: Anti-Src Family (WTAPE) View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Mouse View All»
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