货号：05-1050

品牌：Merck Millipore

规格：100UL

目录价：￥6588.00

市场价格：￥5599.80

会员价格：￥5270.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: 4G10® Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) View All» Promotional Text: FREE Positive Control Included:EGF-stimulated A431 cell lysate (cat. # 12-302)Special Offer on Antibodies! Click Here! View All» Trade Name: Upstate (Millipore) View All» Specificity: Tyrosine-phosphorylated proteins from all species View All» Molecular Weight: Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. View All» Immunogen: 4G10® used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein View All» Modifications: Phosphorylation View All» Isotype: IgG2b View All» Background Information: The development of the anti-phosphotyrosine, clone 4G10® in 1989 was a monumental discovery for researchers. 4G10® was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10® is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10® with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10® Platinum. PY20 itself is a very poor substitute for 4G10®, but its additive effect allow for a greater level of detection on more substrates that even 4G10® alone was not capable of. View All» Species Reactivity: All View All» Application Notes: Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer. View All» Control: Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis. View All» Quality Assurance: Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells. View All» Purification Method: Protein G-Sepharose™ chromatography View All» Presentation: Proprietary mixture of protein G purified mouse monoclonals 4G10® (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide. Liquid at 2-8°C. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Western Blotting

• Immunocytochemistry

• ELISA

• Immunohistochemistry

• Immunofluorescence

• Flow Cytometry

• Immunoprecipitation
  View All» Brand Family: Upstate View All» Product Name: Anti-Phosphotyrosine, 4G10^® Platinum View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Mouse View All»
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