货号：05-1028

品牌：Merck Millipore

规格：

目录价：￥3346.00

市场价格：￥2844.10

会员价格：￥2676.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2 | 05-1028 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Detects MAPKAP Kinase 2 only when phosphorylated on Thr334. View All» Molecular Weight: ~50 kDa View All» Epitope: Thr334 View All» Immunogen: KLH-conjugated synthetic peptide containing the sequence and surrounding amino acids phosphorylated Thr334 of human, mouse, rat, bovine and chicken MAPKAP Kinase 2. Immunizing peptide shared 66% homology with phospho-MAPKAP-K3 at Thr313. View All» Modifications: Phosphorylation View All» Clone: 9F10.2 View All» Isotype: IgG1κ View All» Background Information: MAPKAP Kinases are Ser/Thr kinases activated by phosphorylation by the SAPK2/3, or p38 family of stress-activated kinases. MAPKAP Kinase 2 and 3 phosphorylate substrates with the sequence H-X-R-X-X-S where H is any hydrophobic residue, including CREB (Ser 133) and HSP27. MAPKAP kinases are similar to Rsk kinases, which are activated by mitogen-signaling pathways, rather than stress-signaling pathways. MAPKAP Kinase 2 is involved in many cellular processes that include inflammation, stress, and prolifertion. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, Mouse, Rat. Other species have not been tested. View All» Application Notes: Immunofluorescence Confocal Microscopy Analysis:NIH/3T3 cells were fixed, permeabilized, and stained using Anti-phospho-MAPKAPK2 (Thr334), clone 9F10.2 (Red). Cells were co-stained for actin and nuclear staining with Phalloidin, AlexaFluor® 488 conjugate (Green) and DAPI (Blue), respectively View All» Control: HeLa nuclear extract View All» Quality Assurance: Routinely evaluated by Western BlotWestern Blot Analysis: This lot detected MAPKAPK2 when phosphorylated on Thr334 at 1:1,000 dilution in PC12 lysates resolved via SDS-PAGE and transferred to PVDF (Immobilon-P). View All» Purification Method: Protein G purfied View All» Presentation: Protein G purified mouse monoclonal in PBS with 0.05% NaN3. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance View All» UniProt Number: P49137 View All» Entrez Gene Number: NP_116584 View All» Gene Symbol: MAPKAPK-2 View All» Alternate Names:
  • MAPK-activated protein kinase 2

  • MAPKAP kinase 2

  • Mitogen-activated protein kinase-activated protein kinase 2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunofluorescence

    • ELISA

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue By similarity. Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.Catalytic activity ATP + a protein = ADP + a phosphoprotein.Enzyme regulation Seems to be activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors, the second, triggered by stress and heat shock, depends on the activation of MPK2 and upstream MAPKK/MAPKKK.SUBUNIT: structure Interacts with PHC2.SPECIFITY: Expressed in all tissues examined.PTM:Phosphorylated and activated by MAP kinase.SIMILARITY: Belongs to the protein kinase superfamily. CAMK View All» Product Name: Anti-phospho-MAPKAPK2 (Thr334) Antibody, clone 9F10.2 | 05-1028 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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