货号：04-966

品牌：Merck Millipore

规格：

目录价：￥3346.00

市场价格：￥2844.10

会员价格：￥2676.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cby1 Antibody, clone 8-2 | 04-966 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Cby1. View All» Molecular Weight: ~14 kDa View All» Epitope: Unknown View All» Immunogen: Recombinant protein corresponding to human Cby1. View All» Clone: 8-2 View All» Isotype: IgG2aκ View All» Background Information: Chibby (Cby) is a β-catenin antagonist that is evolutionarily conserved from fly to human. Cby interacts with β-catenin in a manner that competes with TCF/LEF, and thus represses β-catenin-mediated gene activation. Cby and 14-3-3 protein form a trimolecular complex with β-catenin. Cby and 14-3-3 collaborate to promote cytoplasmic localization of β-catenin and thereby inhibit β-catenin signalling. Cby acts, in concert with 14-3-3 proteins, to facilitate nuclear export of β-catenin and thereby antagonize β-catenin signalling. Therefore, the inhibition of β-catenin signalling by Cby involves at least two distinct molecular mechanisms: competing with TCF/LEF transcription factors for β-catenin in the nucleus and facilitating nuclear export of β-catenin through interaction with 14-3-3 following its phosphorylation by AKT/PKB. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Proven to react with human and mouse. View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cby1 in NIH/3T3, A431, HeLa, and C2C12 cells.Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Feng-Qian, L., et al. (2008). J. Cell Biol. 181(7):1141–1154.)Western Blot Analysis: Western Blot is difficult to detect on endogenous lysates but has been shown to work in over expressed cell lines. View All» Control: NIH/3T3, A431, HeLa, and C2C12 cells View All» Quality Assurance: Evaluated by Immunocytochemistry Analysis on NIH/3T3 cells. Immunocytochemistry Analysis: This antibody positively stains the cytoplasm. The nucleus is also stained with less intensity. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q9Y3M2 View All» Entrez Gene Number: NP_056188.1 View All» Gene Symbol:
  • CBY1

  • ARB1

  • CBY

  • Cby

  • Chibby

  • PGEA1

  • PIGEA-14

  • PIGEA14

  • arb1
    View All» Alternate Names:
    • ARPP-binding protein

    • Cytosolic leucine-rich protein

    • PKD2 interactor, Golgi and endoplasmic reticulum-associated 1

    • PKD2 interactor, golgi and endoplasmic reticulum associated 1

    • chibby homolog 1 (Drosophila)

    • chromosome 22 open reading frame 2

    • coiled-coil protein PIGEA-14 2 polycystin-2 interactor, Golgi- and endoplasmic reticulum-associated protein, 14 kDa
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunoprecipitation
        View All» Entrez Gene Summary: Beta-catenin is a transcriptional activator and oncoprotein involved in the development of several cancers. The protein encoded by this gene interacts directly with the C-terminal region of beta-catenin, inhibiting oncogenic beta-catenin-mediated transcriptional activation by competing with transcription factors for binding to beta-catenin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Inhibits the Wnt/Wingless pathway by binding to beta-catenin and inhibiting beta-catenin-mediated transcriptional activation through competition with TCF/LEF transcription factors. Has also been shown to play a role in regulating the intracellular trafficking of polycystin-2/PKD2 and possibly of other intracellular proteins. Promotes adipocyte and cardiomyocyte differentiation.SUBUNIT STRUCTURE: Homodimer; interacts with polycystin-2/PKD2 and GM130. Interacts with the C-terminal region of beta-catenin. SUBCELLULAR LOCATION: Nucleus speckle. Golgi apparatus › trans-Golgi network. Note: Nuclear, in a punctate manner. Also found in the trans-Golgi. TISSUE SPECIFICITY: Widely expressed. Expressed at higher levels in heart, skeletal muscle, kidney and placenta. Also found in brain, lung, liver and testis. Significantly down-regulated in thyroid and metastatic uterine tumors. MISCELLANEOUS: Chibby is Japanese for 'small'; the gene was so named for the RNAi phenotype seen in flies.SEQUENCE SIMILARITIES: Belongs to the chibby family. View All» Product Name: Anti-Cby1, clone 8-2 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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