货号：MAB5404

品牌：Merck Millipore

规格：100Ug

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nitrotyrosine Antibody, clone 2A8.2 | MAB5404 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts with nitrotyrosine. View All» Immunogen: Nitrated KLH. View All» Clone: 2A8.2 View All» Isotype: IgG2b View All» Background Information: The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten. View All» Species Reactivity: All View All» Application Notes: Immunohistochemistry: 1:50-1:100 on paraffin embedded of a previous lot, 10% neutral buffer formalin fixed mouse liver (animal treated with Zymosan) and normal human cerebellum tissues. The tissues must be pretreated with heat induced epitope retrieval (HIER). Incubation with the MAB5404 was for 10 minutes and the reaction was developed with the Chemicon IHC-Select, HRP-DAB kit (Catalog number DAB150).Optimal working dilutions must be determined by end user. View All» Control: Nitrated BSA View All» Quality Assurance: Evaluated by Western Blot on Nitrotyrosine Immunoblot Controls.Western Blot Analysis: 1:500 dilution of this antibody detected Nitrotyrosine on 10 μg of Nitrotyrosine Immunoblot Controls (cat. # 12-354). View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG2b liquid in buffer containing 0.1% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunohistochemistry (Paraffin)

• Western Blotting
  View All» Brand Family: Chemicon View All» Product Name: Anti-Nitrotyrosine Antibody, clone 2A8.2 | MAB5404 View All» Concentration: 1 .0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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