货号：MAB5402

品牌：Merck Millipore

规格：

目录价：￥4383.00

市场价格：￥3725.55

会员价格：￥3506.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Dynamin 1 Antibody, neuronal specific, clone D5 | MAB5402 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Reacts Dynamin 1. Microtubule associated protein View All» Epitope: neuronal specific View All» Immunogen: Recombinant His-tagged fusion protein, corresponding to amino acids 1-750 of Human Dynamin 1. View All» Clone: D5 View All» Isotype: Ig View All» Background Information: Dynamin is a 100 kDa GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homologue, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this complex. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific, Dynamin 2 is ubiquitously expressed and Dynamin 3 localizes to testis, brain and lung. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Western blot on human, rat and mouse brain homogenate: Brains were isolated rodents and homogenized at 2,800 rpm with a Potter-Elvehjem type homogenizer after adding 3 vol of 4°C Buffer A (10 mM Hepes, pH 7.2, 25 mM sucrose, and 10 mM EGTA) containing protease inhibitors (1 mM phenylmethylsulfonyl fluoride, 100 μg/ml soybean trypsin inhibitor, 10 μg/ml leupeptin, 10 μg/ml p-toluenesulfonyl-L-arginine methyl ester, and 5 μg/ml pepstatin A). The homogenate was centrifuged 15 min at 13,000 g then 1 h at 100,000 g, and the supernatant was collected as crude synaptosomal fraction. This fraction was subjected to SDS-PAGE and Western blot analysis. Detects a band of approximately 100 kDa (97.4kDa). Immunofluorescence on rat brain tissue that has been perfused and fixed with formaldehyde, 7 micron cut sections frozen sections. Has not been tested on paraffin embedded tissue sections. Immunoprecipitation on Triton X-100 extracts of post-nuclear supernatant of rat brain homogenate: brains from male Sprague Dawley rats were homogenized as described above in 4 vol of 4°C RIPA buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.5% deoxycholate, and 0.1% SDS) containing protease inhibitors. The homogenate was centrifuged as above and the supernatant collected as the clarified extract. Appropriate antibodies (5 μg) were incubated with 1-ml fractions (15-25 mg) of the clarified extracts, and then preswollen protein G beads (5 mg) were added before a second incubation. Incubations were 2-12 h at 4°C with rotation. Beads and immune complexes were collected by centrifuging 3 min at 7,000 g and then rinsed 4 × 5 min with the starting buffer before being subjected to SDS-PAGE and Western blot analysis. Optimal working dilutions must be determined by end user. View All» Presentation: Purified immunoglobulin. Liquid containing 0.1% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap. View All» UniProt Number: Q05193 View All» Entrez Gene Number:
  • NM_001005336.1

  • NM_004408.2
    View All» Gene Symbol:
    • DNM1

    • dynamin

    • dynamin1

    • Dynamin-1

    • DNM

    • EC 3.6.5.5
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry
        View All» Entrez Gene Summary: This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. View All» UniProt Summary: FUNCTION: SwissProt: Q05193 # Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.SIZE: 864 amino acids; 97407 Da SUBUNIT: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity).SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated.SIMILARITY: SwissProt: Q05193 ## Belongs to the dynamin family. & Contains 1 GED domain. & Contains 1 PH domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Dynamin 1 Antibody, neuronal specific, clone D5 | MAB5402 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Target / Localization: Presynaptic View All» Format: Purified View All» Host: Mouse View All»
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