货号：MAB5304

品牌：Merck Millipore

规格：1EA

目录价：￥5141.24

市场价格：￥4370.05

会员价格：￥4112.99

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Glutamate Antibody | MAB5304 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Glutamate The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity Glutamate-G-BSA 1 Aspartate-G-BSA 1/100,000 GABA-G-BSA 1/100,000 Glutamate 1/100,000 Abbreviations: (G) Glutaraldehyde (BSA) Bovine Serum Albumin NOTE: Antibody reactivity REQUIRES glutaraldehyde fixation thus some glutaraldehyde (0.5%-2.0%) needs to be included in the tissue fixation procedure inorder for the proper reactivity. View All» Immunogen: Glutamate-Glutaraldehyde-BSA. View All» Isotype: IgM View All» Species Reactivity: Rat View All» Application Notes: Immunohistochemistry: 1:2,500-1:10,000 using free floating sections by the PAP technique on rat hippocampus. Optimal working dilutions must be determined by end user. PROTOCOL for Glutamate Detection by Immunohisto/cytochemistry. Example for a rat brain. 1. SOLUTIONS TO BE PREPARED - Solution must be prepared as needed. Solution A: 0.1M cacodylate, 10g/L sodium metabisulfite, pH 6.2. Solution B: 0.1M cacodylate, 10g/L sodium metabisulfite, 3-5% glutaraldehyde, pH 7.5. 2. RAT PERFUSION - The rat is anaesthetized with sodium pentobarbital or Nembutal and perfused intracardially through the aorta using a pump with Solution A (30 mL): 150-300 mL/min, Solution B (500 mL): 150-300 mL/min. 3. POST FIXATION: 15 to 30 minutes in Solution B, then 4 soft washes in 0.05M Tris with 8.5 g/L sodium metabisulfite, pH 7.5 (Solution C) . 4. TISSUE SECTIONING: Vibratome or cryostat sections can be used. 5. REDUCTION STEP: Sections are reduced with Solution C containing 0.1M sodium borohydride for 10 minutes. The sections are washed 4 times in solution C without sodium borohydride. 6. APPLICATION OF GLUTAMATE ANTIBODY: Use a final dilution of 1:2,500-1:10,000 in Solution C containing 0.1% Triton X100 and 2% non-specific serum. Incubate 12 sections per 2 mL diluted antibody overnight, +2-8°C. Then wash the sections three times for 10 minutes each in Solution C. (Note that the antibody may be usable at a higher dilution. This should be explored to minimize the possibility of high background. Additionally, note that a change in the buffering system as indicated in the protocol may change the background and antibody recognition). The specific reaction is then revealed by PAP procedure. 7. SECOND ANTIBODY: Incubate the sections with a 1:50 to 1:200 dilution of goat anti-mouse in Solution B containing 1% non-specific serum for either 3 hrs at 20°C or 1-2 hr at 37°C. Then wash the sections, 3 times, for 10 minutes each with Solution C. 8. PAP: Incubate the sections with the appropriate dilution of peroxidase anti-peroxidase (for free floating method) in Solution C for 1-2 hours at 37°C. Then wash sections 3 times for 10 min each in solution C. 9. VISUALIZATION: The antigen-antibody complexes are visualized using DAB-4-HCl (25 mg/100 mL) (or other chromogen) in 0.05M Tris and filtrated; 0.05% hydrogen peroxide is added. Incubate the sections for 10 minutes at room temp. Stop the reaction by transferring the sections to 5 mL 0.05M Tris. Mount sections on chrome-alum coated slides. Dry overnight at 37°C. Rehydrate sections using conventional histological procedures. Coverslip using rapid mounting media. For research use only; not for use as a diagnostic. View All» Presentation: Liquid in PBS containing 10mM sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 6 months after date. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry View All» Brand Family: Chemicon View All» Product Name: Anti-Glutamate Antibody | MAB5304 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Target / Localization: Presynaptic View All» Format: Purified View All» Host: Mouse View All»