货号：MAB5262

品牌：Merck Millipore

规格：50Ug

目录价：￥5974.00

市场价格：￥5077.90

会员价格：￥4779.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Neurofilament 200 kDa Antibody, clone RT97 | MAB5262 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antibody recognizes an epitope present on human neurofilaments 200 kDa and both bovine 160 kDa and 200 kDa neurofilaments (2). lt reacts with numerous neurites in human senile plaques and decorates neurofibrillary tangles (NFT) (i.e. on the pyramidal cells of the hippocampus and on neurons of the cerebral cortex of subjects with senile dementia of the Alzheimer’s type). View All» Molecular Weight: 200 kDa View All» Immunogen: Rat brain protein preparation View All» Clone: RT97 View All» Isotype: IgG1 View All» Background Information: Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the Millipore Technical Support section. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human and mouse. Expected to react with rat based on sequence homology. View All» Application Notes: Immunohistochemistry: 1-10 μg/mLImmunohistochemistry(paraffin): Representative images from a previous lot.Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Human CerebellumOptimal working dilutions must be determined by end user. View All» Control:
  • Brain

  • Cultured neurons
    View All» Quality Assurance: Evaluated by Western Blot on Mouse brain membrane lysates.Western Blotting Analysis: 1:500 dilution of this antibody detected NEUROFILAMENT on 10 µg of Mouse brain membrane lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» UniProt Number: P12036 View All» Entrez Gene Number: NM_021076.2 View All» Gene Symbol:
    • NEFH

    • NFH

    • NF-H

    • KIAA0845
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» UniProt Summary: FUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.SIZE: 1026 amino acids; 112480 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family. View All» Brand Family: Chemicon View All» Product Name: Anti-Neurofilament 200 kDa Antibody, clone RT97 | MAB5262 View All» Concentration: 1 .0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 μg View All» Format: Purified View All» Host: Mouse View All»
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