货号：MAB5254

品牌：Merck Millipore

规格：40Ug

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Neurofilament 160 kDa Antibody, clone NN18 | MAB5254 View All» Replaces: CBL214 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: The antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984). View All» Molecular Weight: 160 kDa View All» Immunogen: Purified neurofilament polypeptides. View All» Clone: NN18 View All» Isotype: IgG1 View All» Background Information: Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section. View All» Species Reactivity:

• Chicken

• Guinea Pig

• Mouse

• Pig

• Rat

• Human
  View All» Species Reactivity Note: Expected to react with human based on sequence homology. View All» Application Notes: Immunohistochemistry: 5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details. Western Blot: 1:1000. 7.5%-10% SDS-PAGE Optimal working dilutions and protocols must be determined by end user. View All» Control:
  • Brain

  • Cultured neurons
    View All» Quality Assurance: Routinely evaluated by Western Blot on PC12 lysates.Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. View All» Storage Conditions: Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt. Do not freeze. View All» UniProt Number: P07197 View All» Entrez Gene Number: NM_001105541.1 View All» Gene Symbol:
    • NEFM

    • NF-M

    • NEF3

    • NFM
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» UniProt Summary: FUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.SIZE: 916 amino acids; 102448 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family. View All» Brand Family: Chemicon View All» Product Name: Anti-Neurofilament 160 kDa Antibody, clone NN18 | MAB5254 View All» Concentration: 1 .0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 40 µg View All» Format: Purified View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://16ao.com/thread-131852.htm
        © Merck Millipore代理MAB5254 Anti-Neurofilament 160 kDa Antibody, clone NN18;store at +2℃ to +8℃，产品报价联系微信jinshanbio