货号：MAB5234

品牌：Merck Millipore

规格：50Ug

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Neurocan Antibody | MAB5234 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Neurocan, chicken. Recognizes the middle region of neurocan. View All» Immunogen: Mixture of chicken chondroitin sulfate proteoglycans. View All» Isotype: IgG1 View All» Background Information: Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration. View All» Species Reactivity: Chicken View All» Application Notes: Western blot: Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan. Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples. Immunoprecipitation: 1 μg/mL ELISA: 1 μg/mL, excellent for core protein, good for monomer Immunocytochemistry: not tested Immunohistochemistry: does not work on fixed samples, unfixed has not been tested. Optimal working dilutions must be determined by the end user. View All» Presentation: Purified immunoglobulin. Liquid in PBS. Contains no preservative. View All» Storage Conditions: Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: O14594 View All» Entrez Gene Number: NM_004386.2 View All» Gene Symbol:

• NCAN

• FLJ44681

• NEUR

• CSPG3
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Immunoprecipitation

  • Western Blotting
    View All» Entrez Gene Summary: Neurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: O14594 # May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.SIZE: 1321 amino acids; 143093 Da TISSUE SPECIFICITY: Brain.SIMILARITY: SwissProt: O14594 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain. View All» Brand Family: Chemicon View All» Product Name: Anti-Neurocan Antibody | MAB5234 View All» Concentration: 1.2 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Purified View All» Host: Mouse View All»
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