货号：04-403

品牌：Merck Millipore

规格：

目录价：￥3662.00

市场价格：￥3112.70

会员价格：￥2929.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PI3 Kinase Antibody, p85α Rabbit | 04-403 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes the C-terminal domain of PI3 Kinase, p85α subunit. View All» Molecular Weight: 85 kDa View All» Epitope: C-terminus View All» Immunogen: KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p85α subunit. View All» Isotype: IgG View All» Background Information: PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Proven to react with human, mouse and rat. Reactivity with other species has not been confirmed. View All» Application Notes: Immunoprecipitation: A 1:50 dilution of a previous lot of this antibody immunoprecipitated PI3K, p85α using protein G:agarose beads.Flow Cytometry: A 1:50 dilution of a previous lot of this antibody stained cells for intracellular staining for flow cytometry analysis. View All» Control: Lysates from A431, MCF-7 or Raji cells View All» Quality Assurance: Routinely evaluated by Western Blot on A431 lysate. Western Blot Analysis:A 1:2,000 dilution of the lot detected endogenous PI3K, p85α in lysates from A431 cells. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage. View All» UniProt Number: P27986 View All» Entrez Gene Number: NM_181504.2 View All» Gene Symbol:
  • PIK3R1

  • GRB1

  • PI3K

  • p85-ALPHA
    View All» Alternate Names:
    • PI3-kinase p85 subunit alpha

    • PtdIns-3-kinase p85-alpha

    • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85alpha)

    • phosphatidylinositol 3-kinase, regulatory, 1

    • phosphatidylinositol 3-kinase-associated p-85 alpha

    • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

    • phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)

    • phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85alpha).
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Flow Cytometry

      • Immunocytochemistry

      • Immunofluorescence

      • Immunohistochemistry (Paraffin)

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. View All» UniProt Summary: FUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.SIZE: 724 amino acids; 83598 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1.TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance.SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. View All» Brand Family: Upstate View All» Product Name: Anti-PI3 Kinase Antibody, p85α Rabbit | 04-403 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»
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