货号：04-334

品牌：Merck Millipore

规格：

目录价：￥3615.00

市场价格：￥3072.75

会员价格：￥2892.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Cbl (Tyr774) Antibody, rabbit | 04-334 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes Cbl when phosphorylated on tyrosine 774. View All» Molecular Weight: 120 kDa View All» Immunogen: KLH-conjugated synthetic peptide corresponding to the amino acid residues surrounding tyrosine 774 of human Cbl. View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Species Reactivity: Human View All» Non-Reactive Species:

• Mouse

• Rat
  View All» Application Notes: Western Blot: 1:100 on lysates from pervanadate-treated Jurkat cells. Immunoprecipitation: 1:20. Flow Cytometry: 1:20. Permeablization of cells is required. Immunohistochemistry: 1:25 on paraffin-embedded sections of human cervical carcinoma. Immunofluorescence: 1:25. Paraformaldehyde fixation recommended. Optimal working dilutions must be determined by the end user. View All» Control: POSITIVE: Pervanadate-treated Jurkat cells. View All» Quality Assurance: Routinely evaluated by immunoblot analysis. View All» Presentation: Rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol. View All» Storage Conditions: 2 years at -20°C from date of shipment. View All» UniProt Number: P22681 View All» Entrez Gene Number: NM_005188.2 View All» Gene Symbol:
  • CBL

  • CBL2

  • RNF55

  • C-CBL
    View All» Alternate Names: Cas-Br-M (murine) ecotropic retroviral transforming sequence View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Flow Cytometry

    • Immunofluorescence

    • Immunoprecipitation

    • Western Blotting

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. View All» UniProt Summary: FUNCTION: SwissProt: P22681 # Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.SIZE: 906 amino acids; 99647 Da SUBUNIT: Associates with NCK via its SH3 domain. The phosphorylated C-terminus interacts with CD2AP via its second SH3 domain. Binds to UBE2L3. Interacts with adapters SLA, SLA2 and with the phosphorylated C-terminus of SH2B2. Interacts with EGFR, SYK and ZAP70 via the highly conserved Cbl-N region. Also interacts with SORBS1 and INPPL1/SHIP2. Interacts with phosphorylated LAT2. May interact with CBLB (By similarity).SUBCELLULAR LOCATION: Cytoplasm.DOMAIN: SwissProt: P22681 The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. & The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.PTM: Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.DISEASE:SwissProt: P22681 # Can be converted to an oncogenic protein by deletions or mutations that disturb its ability to down-regulate RTKs.SIMILARITY: Contains 1 CBL N-terminal domain. & Contains 2 EF-hand-like domains. & Contains 1 RING-type zinc finger. & Contains 1 SH2 domain. & Contains 1 UBA domain.MISCELLANEOUS: This protein has one functional calcium-binding site. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Cbl (Tyr774) Antibody, rabbit | 04-334 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»
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