货号：04-239

品牌：Merck Millipore

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目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-p16/INK4a Antibody, clone EP435Y | 04-239 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes p16/INK4a. View All» Molecular Weight: 16 kDa View All» Immunogen: Recombinant p16/INK4a human protein View All» Clone: EP435Y View All» Isotype: IgG View All» Background Information: p16 (INK4a, CDKN2A, cyclin-dependent kinase inhibitor 2A) is a tumor suppressor gene that is important in regulating cell cycle. Mutations in p16 can result in various cancers, most notably melanoma. p16 is a splice variant that comes from the gene CDKN2A. CDKN2A gene produces 2 major proteins, p16 (INK4) and p14 (ARF), which binds MDM2. View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with human. Not expected to react with mouse and rat View All» Application Notes: Immunoprecipitation: A 1:50 dilution of a previous lot was used in IP.Flow Cytometry: A 1:10 dilution of a previous lot was used in FC. View All» Control: HEK293 whole cell lysate. View All» Quality Assurance: Routinely evaluated by Western Blot on HEK293 whole cell lysate.Western Blot Analysis: A 1:500-1:2,000 dilution of this lot was used to detect p16/INK4a HEK293 whole cell lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P42771 View All» Entrez Gene Number: NM_000077.3 View All» Gene Symbol:

• ARF

• CDK4I

• CDKN2

• CMM2

• INK4

• INK4a

• MLM

• MTS-1

• MTS1

• P16

• TP16

• p14

• p14ARF

• p16

• p16-INK4

• p16-INK4a
  View All» Alternate Names:
  • CDK4 inhibitor p16-INK4

  • Cyclin-dependent kinase 4 inhibitor A

  • Multiple tumor suppressor 1

  • cell cycle negative regulator beta

  • cyclin-dependent kinase inhibitor 2A

  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)

  • cyclin-dependent kinase inhibitor p16
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Flow Cytometry

    • Immunoprecipitation
      View All» Entrez Gene Summary: This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq] View All» UniProt Summary: Function: Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. Ref.10Subunit structure: Heterodimer with CDK4 or CDK6. Isoform 3 does not bind to CDK4.Tissue specificity: Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. Ref.2Polymorphism: Genetic variations in CDKN2A may underlie susceptibility to uveal melanoma [MIM:155720]. Uveal melanoma is the most common type of ocular malignant tumor, consisting of overgrowth of uveal melanocytes and often preceded by a uveal nevus.Involvement in disease: Defects in CDKN2A are involved in tumor formation in a wide range of tissues.Defects in CDKN2A are the cause of cutaneous malignant melanoma 2 (CMM2) [MIM:155601]. Inheritance is autosomal dominant. Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a preexisting benign nevus, which occurs most often in the skin but also may involve other sites. Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC) [MIM:606719].Defects in CDKN2A are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Defects in CDKN2A are the cause of melanoma-astrocytoma syndrome [MIM:155755]. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. Sequence similarities: Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.Contains: 4 ANK repeats. View All» Product Name: Anti-p16/INK4a, clone EP435Y View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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