货号：04-227

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Estrogen Receptor α Antibody, clone E115 | 04-227 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes Estrogen Receptor α. View All» Molecular Weight: 67 kDa View All» Immunogen: Synthetic peptide directed against human Estrogen Receptor α. View All» Clone: E115 View All» Isotype: IgG View All» Background Information: Estrogen Receptor α (ER alpha) is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Proven to react with human, mouse and rat. View All» Application Notes: Immunohistochemistry:Immunocytochemistry: 1:100 dilution of a previous lot was used on immunocytochemistry. View All» Control: MCF-7 cell lysate. View All» Quality Assurance: Routinely evaluated by western blot on MCF-7 cell lysate.Western Blot Analysis:A 1:250-1:500 dilution of this lot was used to detect Estrogen Receptor  in MCF-7 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P03372 View All» Entrez Gene Number: NP_000116.2 View All» Gene Symbol:
  • ER

  • ER-alpha

  • ESR

  • ESRA

  • Era

  • NR3A1

  • DKFZp686N23123

  • OTTHUMP00000017718

  • OTTHUMP00000017719
    View All» Alternate Names:
    • Estradiol receptor

    • Nuclear receptor subfamily 3 group A member 1

    • estrogen receptor 1

    • estrogen receptor alpha

    • oestrogen receptor

    • steroid hormone receptor
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important forhormone binding, DNA binding, and activation of transcription. Theprotein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breastcancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.SIZE: 595 amino acids; 66216 Da SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.SUBCELLULAR LOCATION: Nucleus.DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites. View All» Brand Family: Upstate View All» Product Name: Anti-Estrogen Receptor α, clone E115 Antibody View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Culture Supernatant View All» Host: Rabbit View All»
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