货号：04-1555

品牌：Merck Millipore

规格：

目录价：￥4239.00

市场价格：￥3603.15

会员价格：￥3391.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Mdmx Antibody, clone 8C6 | 04-1555 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Mdmx. View All» Molecular Weight: ~ 80 kDa View All» Epitope: Unknown View All» Immunogen: His-tagged recombinant protein corresponding to human Mdmx. View All» Clone: 8C6 View All» Isotype: IgG2bκ View All» Background Information: Mdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx. View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with human. View All» Application Notes: Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)Immunohistochemistry Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)Immunoprecipitation Analysis: A representative lot was used by an independent laboratoryfor this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)Flow Cytometry Analysis: A previous lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.) View All» Control: MCF-7 cell lysate View All» Quality Assurance: Evaluated by Western Blot in MCF-7 cell lysate.Western Blot Analysis: 0.5 µg/ml of this antibody detected Mdmx on 10 µg of MCF-7 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O15151 View All» Entrez Gene Number: NP_002384.2 View All» Gene Symbol:

• HDMX

• MDMX

• MRP1

• MDM4
  View All» Alternate Names:
  • Double minute 4 protein

  • MDM4-related protein 1

  • Mdm2-like p53-binding protein

  • Mdm4 p53 binding protein homolog (mouse)

  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein

  • Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)

  • Protein Mdmx

  • double minute 4, human homolog of

  • p53-binding protein

  • mouse double minute 4 homolog

  • mouse double minute 4, human homolog of

  • p53-binding protein

  • p53-binding protein 2 p53-binding protein Mdm4
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation

    • Immunocytochemistry

    • Immunohistochemistry

    • Flow Cytometry
      View All» Entrez Gene Summary: The human MDM4 gene(encodes Mdmx protein), which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions.SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: In all tissues tested, with high levels in thymus.DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.Contains 1 RanBP2-type zinc finger.Contains 1 RING-type zinc finger.Contains 1 SWIB domain.MASS SPECTROMETRY: Molecular mass is 54863.3 Da from positions 1 - 490. Determined by MALDI. View All» Product Name: Anti-Mdmx, clone 8C6 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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