货号：04-1540

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PICH Antibody, clone 142-26-3 | 04-1540 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Plk1-interacting checkpoint helicase. View All» Molecular Weight: ~160 kDa observed View All» Epitope: Unknown View All» Immunogen: Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase. View All» Clone: 142-26-3 View All» Isotype: IgG1κ View All» Background Information: Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. View All» Control: HeLa-S3 cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa-S3 cell lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q2NKX8 View All» Entrez Gene Number: NP_060139 View All» Gene Symbol:

• PICH

• ERCC6L

• FLJ20105

• MGC131695
  View All» Alternate Names:
  • excision repair cross-complementing rodent repair deficiency, complementation group 6-like

  • excision repair cross-complementing rodent repair deficiency complementation group 6 - like

  • Tumor antigen BJ-HCC-15

  • PLK1-interacting checkpoint helicase

  • ATP-dependent helicase ERCC6-like

  • excision repair protein ERCC6-like

  • SNF2/RAD54 family protein

  • DNA excision repair protein ERCC-6-like, PLK1-interacting checkpoint helicase, Tumor antigen BJ-HCC-15
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation

    • Immunocytochemistry
      View All» UniProt Summary: FUNCTION: DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. SUBUNIT STRUCTURE; Interacts with PLK1, which phosphorylates it. Both proteins are mutually dependent on each other for correct subcellular localization. SUBCELLULAR LOCATION: Centromere. Kinetochore. Note: Localizes to kinetochores, inner centromeres and thin threads connecting separating chromosomes even during anaphase. In prometaphase cells, it mostly concentrates in between kinetochores. In metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes and are composed of catenated centromeric DNA. Evolution from inner centromeres to thin threads takes place in response to tension. Resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset. PTM: Phosphorylation by PLK1 prevents the association with chromosome arms and restricts its localization to the kinetochore-centromere region.SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 2 TPR repeats.SEQUENCE CAUTION: The sequence AAM82750.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys.The sequence BAA90952.1 differs from that shown. Reason: Erroneous termination at position 803. Translated as Lys. View All» Product Name: Anti-PICH, clone 142-26-3 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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