货号：04-1497

品牌：Merck Millipore

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目录价：￥4891.00

市场价格：￥4157.35

会员价格：￥3912.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CTCF Antibody, clone 2.27.2.5.3.2.5 | 04-1497 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes CTCF. View All» Molecular Weight: ~ 83 kDa Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with different molecular weight on Western Blot. Modifications are dependent on cell type and growing condition.The following molecular weights have been reported in the literature: 180 kDa (CTCF-180), 130 kDa (CTCF-130), 82 kDa (CTCF-82), 70 kDa (CTCF-70). See Klenova,E.M.,et al, (1997) Nucleic Acids Res. 25, 466–473 and Torrano,V,et al, (2006) Journal of Cell Science 119, 1746-1759) for additional details. View All» Epitope: Unknown View All» Immunogen: His-tagged recombinant protein corresponding to human CTCF. View All» Isotype: IgG1 View All» Background Information: CTCF, also known as CCCTC-binding factor, contains 11 zinc finger (ZF) binding domains that allow it to bind different DNA target sequences and proteins. This multifunctional protein can act as a transcriptional activator or repressor. As a transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. Mutations of CTCF in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms tumors, suggesting a role for CTCF may as a tumor suppressor. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Pugacheva, E., et al. (2005). Human Molecular Genetics. 14(7):953–965.) View All» Control: K562 cell lysate View All» Quality Assurance: Evaluated by Western Blot in K562 cell lysate.Western Blot Analysis: 0.5 µg/ml of this antibody detected CTCF on 10 µg of K562 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P49711 View All» Entrez Gene Number: NP_006556.1 View All» Gene Symbol: CTCF View All» Alternate Names:

• 11 zinc finger transcriptional repressor

• 11-zinc finger protein

• CCCTC-binding factor

• CCCTC-binding factor (zinc finger protein)

• CTCFL paralog

• transcriptional repressor CTCF
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunoprecipitation

  • Immunohistochemistry
    View All» Entrez Gene Summary: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.r/>SIZE: 727 amino acids; 82785 Da SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes.SIMILARITY: Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers. View All» Product Name: Anti-CTCF, clone 2.27.2.5.3.2.5 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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