货号：04-1160

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-KSR1 Antibody, clone EPR2421Y, Rabbit | 04-1160 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes KSR1. View All» Molecular Weight: ~110 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to residues near the C-terminus of human KSR1. View All» Clone: EPR2421Y View All» Isotype: IgG View All» Background Information: The Kinase Suppressor of Ras (KSR1) is a recently identified participant in the Ras signaling pathway. KSR is capable of uncoupling the MAP kinase activation from its target phosphorylation, and thus provide a novel mechanism for modulating the Ras-MAP kinase signaling pathway. The recently identified, B-KSR, is a related isoform. KSR is thought to phosphorylate and activate Raf-1, protecting against cytokine-mediated apoptosis. Recent studies suggest that KSR interacts with several components of the MAP kinase pathway, including Raf-1, MEK1/2 and ERK1/2. KSR seems to act as a scaffolding protein that coordinates the assembly of a membrane-localized, multiprotein MAP kinase complex, a vital step in Ras-mediated signal transduction. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human and mouse. View All» Application Notes: Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected was used in IHC.Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC. View All» Control: 293 cell lysate View All» Quality Assurance: Evaluated by Western Blot on 293 cell lysates.Western Blot Analysis: A 1:500-2,000 dilution of this antibody was used to detect KSR1 in 293 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q8IVT5 View All» Entrez Gene Number: NP_006305. View All» Gene Symbol:
  • KSR

  • RSU2
    View All» Alternate Names:
    • kinase suppressor of ras

    • kinase suppressor of ras 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene is a necessary element in receptor tyrosine kinase pathways, possibly as a tyrosine phosphorylation target. It is involved in regulation of RAF in the MAPK pathway and may also play a role in a MAPK-independent pathway. View All» UniProt Summary: FUNCTION: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.SUBUNIT STRUCTURE: Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner By similarity. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.SUBCELLULAR LOCATION: Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery By similarity.PTM: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated By similarity. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain. View All» Product Name: Anti-KSR1, clone EPR2421Y, Rabbit Monoclonal Antibody (04-1160) View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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