货号：04-1159

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, Rabbit | 04-1159 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants. View All» Molecular Weight: ~25/57 kDa View All» Epitope: a.a. 236-242 View All» Immunogen: Synthetic peptide corresponding to residues in human LEF-1/TCF-1 α. View All» Clone: EPR2029Y View All» Isotype: IgG View All» Background Information: Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. View All» Species Reactivity: Human View All» Species Reactivity Note: Human View All» Application Notes: Immunohistochemistry (Paraffin) Analysis: A 1:100-250 dilution from a previous lot detected LEF-1/TCF-1 α in human tonsil tissue.Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC. View All» Control: Jurkat cell lysate View All» Quality Assurance: Evaluated by Western Blot on Jurkat cell lysates.Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect LEF-1/TCF-1 α in Jurkat cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q9UJU2 View All» Entrez Gene Number: NM_016269.2 View All» Gene Symbol:

• DKFZp586H0919

• LEF-1

• TCF1-alpha

• TCF1ALPHA
  View All» Alternate Names:
  • T cell-specific transcription factor 1-alpha

  • lymphoid enhancer binding factor-1

  • lymphoid enhancer-binding factor 1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)
      View All» UniProt Summary: FUNCTION: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 By similarity. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. SUBUNIT STRUCTURE: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG By similarity. Binds THOC4, MDFI and MDFIC. SUBCELLULAR LOCATION: Nucleus By similarity. Note: Found in nuclear bodies upon PIASG binding By similarity.TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN: Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.SEQUENCE SIMILARITIES: Belongs to the TCF/LEF family.Contains 1 HMG box DNA-binding domain. View All» Product Name: Anti-LEF-1/TCF-1 α, clone EPR2029Y, Rabbit Monoclonal Antibody (04-1159) View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://16ao.com/thread-131418.htm
      © Merck Millipore代理04-1159 Anti-LEF-1/TCF-1 a, clone EPR2029Y,;已停产，产品报价联系微信jinshanbio