货号：MAB378

品牌：Merck Millipore

规格：100UL

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MAP2A Antibody, 2B | MAB378 View All» Replaces: MAB3418 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: MAP2a & b. Does not recognize other variants. View All» Molecular Weight: ~ 300 kDa View All» Immunogen: Bovine MAP2 View All» Clone: AP20 View All» Isotype: IgG1 View All» Background Information: MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984). MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987). View All» Species Reactivity:

• Amphibian

• Avian

• Bovine

• Human

• Mouse

• Rat

• Xenopus

• Quail
  View All» Application Notes: Immunohistochemistry: 1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.Western blot: SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Western blot: A 1:500 dilution was used in a previous lot.Optimal working dilutions must be determined by end user. View All» Control:
  • Brain tissue

  • Cultured neurons
    View All» Quality Assurance: Routinely evaluated by Western Blot on PC12 lysates.Western Blot Analysis: 1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates. View All» Purification Method: Unpurified View All» Presentation: Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide View All» Storage Conditions: Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P11137 View All» Entrez Gene Number: NM_001039538.1 View All» Gene Symbol:
    • MAP2

    • MAP2B

    • MAP-2

    • MAP2C

    • DKFZp686I2148

    • MAP2A
      View All» Alternate Names: Microtubule Associated Protein 2 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described. View All» UniProt Summary: FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.SIZE: 1827 amino acids; 199539 Da PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats. View All» Brand Family: Chemicon View All» Product Name: Anti-MAP2A Antibody, 2B | MAB378 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Target / Localization: All neurons, cytoplasmic, soma, dendritic View All» Format: Ascites View All» Host: Mouse View All»
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