货号：MAB3747

品牌：Merck Millipore

规格：100Ug

目录价：￥5215.00

市场价格：￥4432.75

会员价格：￥4172.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Microphthalmia (Mi) Antibody, clone C5 | MAB3747 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi). There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells andheart. It reacts with both melanocytic as well as the nonmelanocytic isoforms of Mi. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. View All» Molecular Weight: 53-56 kDa View All» Immunogen: Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells. View All» Clone: C5 View All» Isotype: IgG1 View All» Background Information: MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, while the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of Mi causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3' half of Mi including most of the b-HLH-ZIP region. Osteoclasts from these animals lack Mi protein in contrast to wild-type rat, mouse, and human osteoclasts. View All» Species Reactivity:

• Human

• Rat

• Mouse
  View All» Species Reactivity Note: Expected to react with human and rat based on sequence homology. View All» Application Notes: Immunoprecipitation: A previous lot of this antibody was used in IP. (2 µg/mg of protein lysate)Gel supershift assays: A previous lot of this antibody was used in EMSA.Immunohistochemistry(paraffin): A previous lot of this antibody was used in IH on frozen and formalin/paraffin tissue sections. Optimal working dilutions must be determined by end user. View All» Control: 501 Mel human melanoma cells, wild-type human, rat, mouse osteoclast cells. View All» Quality Assurance: Routinely evaluated by Western Blot on Mouse Brain lysates.Western Blot Analysis: 1:500 dilution of this lot detected Mi on 10 μg of Mouse Brain lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified mouse monoclonal IgG1 in phosphate buffered saline with 0.08% sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG1 and affect product performance. View All» Gene Symbol:
  • LZMS

  • MCOPS1

  • MAA
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Electrophoretic Mobility Shift Assay

    • Immunohistochemistry

    • Immunohistochemistry (Paraffin)

    • Immunoprecipitation

    • Western Blotting
      View All» Brand Family: Chemicon View All» Product Name: Anti-Microphthalmia (Mi) Antibody, clone C5 | MAB3747 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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