货号：04-1088

品牌：Merck Millipore

规格：

目录价：￥4259.00

市场价格：￥3620.15

会员价格：￥3407.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Caspase-3 (Pro) Antibody, clone E83-103, Rabbit | 04-1088 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases. View All» Molecular Weight: ~35 kDa View All» Epitope: N-terminus View All» Immunogen: Synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit). View All» Clone: E83-103 View All» Isotype: IgG View All» Background Information: Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human View All» Application Notes: Immunohistochemistry Analysis: 1:250 dilution from a previous lot detected pro-caspase-3 in human colon adenocarcinoma tissue. View All» Control: Jurkat lysates View All» Quality Assurance: Evaluated by Western Blotting on Jurkat cell lysates.Western Blotting Analysis: 1:500 dilution of this antibody was used to detect pro-caspase-3 in Jurkat cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P42574 View All» Entrez Gene Number: NP_004337.2 View All» Gene Symbol:
  • Apopain

  • CASP-3

  • CPP-32

  • CPP32

  • CPP32B

  • EC 3.4.22.56

  • OTTHUMP00000165054

  • SCA-1

  • Yama

  • apopain

  • procaspase3
    View All» Alternate Names:
    • Cysteine protease CPP32

    • PARP cleavage protease

    • SREBP cleavage activity 1

    • Yama protein

    • caspase 3

    • caspase 3, apoptosis-related cysteine peptidase

    • caspase 3, apoptosis-related cysteine protease
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunohistochemistry (Paraffin)

      • Immunocytochemistry

      • Flow Cytometry
        View All» Entrez Gene Summary: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. CATALYTIC ACTIVITY: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.ENZYME REGULATION: Inhibited by isatin sulfonamides.SUBUNIT STRUCTURE: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.Subcellular location Cytoplasm. TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.SEQUENCE SIMILARITY: Belongs to the peptidase C14A family. View All» Product Name: Anti-Caspase-3 (Pro), clone E83-103, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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